农业生物技术学报2025，Vol.33Issue(1)：157-165,9.DOI:10.3969/j.issn.1674-7968.2025.01.014
MiR-1306-5p对鸡前脂肪细胞增殖与分化的影响
Effect of MiR-1306-5p on the Proliferation and Differentiation of Chicken(Gallus gallus)Preadipocytes
摘要
Abstract
The functional studies of miR-1306-5p are mostly found to affect the proliferation of smooth muscle cells and various tumor cells.In the early stage,the research group collected the pectoral muscles of rose-crowned chickens(Gallus gallus)and Kebao chickens on the 8th day of embryonic stage for high-throughput sequencing.In this study,differentially expressed miR-1306-5p was selected from the previous sequencing data.The aim was to investigate the function of miR-1306-5p in the proliferation and differentiation of chicken preadipocytes.Using Targetscan and miRanda software predicted target genes of miR-1306-5p.Select the target gene related to fat,bone morphogenetic protein 2(BMP2),and design the wild type and mutant vector of the target gene BMP2.The vector and miR-1306-5p mimics were constructed to co-transfect chicken fibroblasts,and the target relationship between them was verified by luciferase reporter gene detection system.miR-1306-5p mimics and inhibitors were transfected into chicken preadipocytes,respectively.Cell proliferation capacity was detected by Cell Counting Kit-8(CCK-8)kits.The differentiation induced by oleic acid was detected by oil red O staining.The results showed that the luciferase activity in the BMP2 wild type co-transfected with miR-1306-5p mimics group was extremely significantly decreased compared with the negative control group(P＜0.01),while the mutant type had no significant change.After transfection with miR-1306-5p mimics for 48,72 and 96 h,the proliferation capacity of chicken preadipocytes was extremely significantly lower than that of the negative control group(P＜0.01).The gene expression of fatty acid binding protein 4(FABP4)was extremely significantly increased during oleic acid-induced differentiation of chicken preadipocytes(P＜0.01).The gene expressions of endogenous miR-1306-5p and BMP2 were extremely significantly decreased at 48 and 72 h after induction of differentiation(P＜0.01).Lipid droplets were smaller after transfection of miR-1306-5p mimics and increased after transfection of miR-1306-5p inhibitor in chicken preadipocytes.Therefore,miR-1306-5p may inhibit the proliferation and differentiation of chicken preadipocytes by targeting BMP2.This study provides basic data for further exploring the mechanism of miR-1306-5p in chicken preadipocyte differentiation.关键词
鸡前脂肪细胞/miR-1306-5p/骨形态发生蛋白2(BMP2)/细胞增殖/细胞分化Key words
Chicken preadipocytes/miR-1306-5p/Bone morphogenetic protein 2(BMP2)/Cell proliferation/Cell differentiation分类
农业科技引用本文复制引用
孙楠桢,张蕾,陈若楠,张若彤,孙杰..MiR-1306-5p对鸡前脂肪细胞增殖与分化的影响[J].农业生物技术学报,2025,33(1):157-165,9.基金项目
国家自然科学基金(31660654) （31660654）
