农业生物技术学报2025，Vol.33Issue(1)：166-178,13.DOI:10.3969/j.issn.1674-7968.2025.01.015
低氧胁迫对虹鳟心脏组织抗氧化和细胞凋亡的影响
Effects of Hypoxia Stress on Antioxidant and Apoptotic in the Heart of Rainbow Trout(Oncorhynchus mykiss)
摘要
Abstract
Rainbow trout(Oncorhynchus mykiss)is extremely sensitive to hypoxia,and its antioxidant capacity and apoptosis are affected by hypoxia environments.To understand the effects of hypoxia stress on the heart of rainbow trout,antioxidant and apoptotic gene expression were measured using enzyme activity assay and qRT-PCR during moderate hypoxia(4.5±0.1 mg/L)and severe hypoxia(3.0±0.1 mg/L)stress for 4,8,12,24 h,1 month,and reoxygenation(8.5±0.1 mg/L)for 12 and 24 h.The results showed that total superoxide dismutase(T-SOD)and catalases(CAT)activities were significantly reduced at 24 h and 1 month,malondialdehyde(MDA)content was significantly increased at 12 h(P＜0.05)under severe hypoxia stress;T-SOD,CAT and total antioxidant capacity(T-AOC)activities were gradually increased under moderate hypoxia stress.Compared with the control group,total protein(TP)content was significantly decreased after 1 month of hypoxia stress(P＜0.05).The expression of antioxidant-related genes Cu/Zn-SOD(cu/zn-sod)and Mn-SOD(mn-sod)were significantly decreased under severe hypoxia and moderate hypoxia stress compared with the control group(P＜0.05);the expression of cat was significantly decreased at 4,8 and 12 h under severe hypoxia(P＜0.05).After severe hypoxia stress,the expression of apoptosis-related gene B-cell lymphoma-2(Bcl-2)increased significantly under short-term hypoxia stress compared with the control group,while the expression of Bcl-2-associated X(Bax)showed no significant difference,and the expression of caspase-3(casp3)and tumor suppressor gene 53(p53)decreased significantly at 12 h(P＜0.05).Acid phosphatase(ACP)and alkaline phosphatase(AKP)were significantly higher than those of the control group at 12 h under severe hypoxia stress and moderate hypoxia stress(P＜0.05).The above results suggested that rainbow trout heart could reduce oxidative damage by regulating the activities of antioxidant enzymes and the expression of apoptosis-related genes under different concentrations of hypoxia.This study provides a theoretical basis for further elucidating the oxidative stress mechanism in rainbow trout heart under hypoxia stress.关键词
虹鳟/低氧/生理生化指标/氧化应激/细胞凋亡Key words
Rainbow trout/Hypoxia/Physiological and biochemical indicators/Oxidative stress/Apoptosis分类
农业科技引用本文复制引用
曾粤,李永娟,黄进强,吴深基,赵璐,孙同振,马九菊..低氧胁迫对虹鳟心脏组织抗氧化和细胞凋亡的影响[J].农业生物技术学报,2025,33(1):166-178,13.基金项目
国家自然科学基金(32060823) （32060823）
甘肃农业大学学科团队项目(GAU-XKTD-2022-23) （GAU-XKTD-2022-23）
