农业生物技术学报2025，Vol.33Issue(1)：208-219,12.DOI:10.3969/j.issn.1674-7968.2025.01.019
高通量低成本检测牛MSTN基因突变的DAVID技术构建
Construction of DAVID Technology for High-throughput and Low-cost Detection of Bovine(Bos taurus)MSTN Gene Mutations
摘要
Abstract
Myostatin(MSTN)is a negative regulator of skeletal muscle growth and development,and mutations in the MSTN gene can promote muscle development in animals.Currently,Sanger sequencing technology is the classic method for detecting MSTN gene mutations,but this method has the disadvantages of low detection throughput,low visualisation of mutation information and high detection cost.In this study,MSTN gene mutations in 96 cattle(Bos taurus)could be detected at one time by using next-generation sequencing technology with a specific Barcode sequence designed.In addition,based on the Barcode sequences in the sequencing Fastq result files,the related data analysis DAVID(detect and visualize insertion-deletion)software was developed using asynchronous threading and concurrent algorithms.Using DAVID software,the sequencing data of 96 mixed samples were split one by one and compared with amplicon sequence,and finally the graphical and statistical information of MSTN gene mutation was obtained.Using DAVID technology,96 Mongolian cattle were examined,and found that 1 head of cattle was heterozygous for 11 bp deletion of the MSTN gene,and the remaining 95 head of cattle were wild-type,and the 11 bp heterozygous deletion was further verified by Sanger sequencing.This study constructed a high-throughput technical system for detecting bovine MSTN gene mutations,and provides technical support for genetic improvement and breeding of cattle.关键词
肌肉生长抑制素(MSTN)/蒙古牛/基因突变/基因型检测Key words
Myostatin(MSTN)/Mongolian cattle/Genetic mutation/Genotyping detection分类
农业科技引用本文复制引用
裴栋超,海超,赵春生,张立果,吴迪,王德政,张鑫怡,杨磊,李光鹏..高通量低成本检测牛MSTN基因突变的DAVID技术构建[J].农业生物技术学报,2025,33(1):208-219,12.基金项目
科技创新2030-重大项目(2023ZD0404803) （2023ZD0404803）
国家自然科学基金(32360837 （）
32341052) （）
内蒙古科技领军团队(2022LJRC0006) （2022LJRC0006）
农业农村部农业重大科技项目(NK202213020303) （NK202213020303）
内蒙古揭榜挂帅项目(2022JBGS0025) （2022JBGS0025）
内蒙古科技重大专项(2021ZD0009 （）
2021ZD0008 （）
2022ZD0008) （）
内蒙古科技计划(2023KJHZ0028) （2023KJHZ0028）
内蒙古青年科技英才项目(NJYT23138) （NJYT23138）
中央引导地方科技发展资金(2022ZY0212) （2022ZY0212）
内蒙古自然科学基金(2023MS03004) （2023MS03004）
呼和浩特市高校院所协同创新(XTCX2023-06) （XTCX2023-06）
内蒙古大学教育部工程中心课题(JYBGCSYS2022) （JYBGCSYS2022）
新疆维吾尔自治区重大科技专项(2023A02011-1) （2023A02011-1）
