岭南现代临床外科2024，Vol.24Issue(5)：263-275,13.DOI:10.3969/j.issn.1009-976X.2024.05.001
TRIM5与恶性肿瘤不良预后相关性及其在胰腺癌恶性表型中的作用研究
Pan-cancer analysis of tripartite motif 5 and its validation in promoting malignant phenotypes of pancreatic cancer
摘要
Abstract
Objective To evaluate the expression level of TRIM5 in pan-cancer and its relationship with prognosis,clinical stage,and immune micro-environment.Methods TRIM5 pan-oncogene expression profile,somatic mutation data,and clinically relevant information were obtained from TCGA,TARGET,and GTEx databases.Use R software package to analyze the relationship between TRIM5 and the progno-sis,clinical pathological characteristics,and genomic heterogeneity of cancer patients.The relationship between TRIM5 and immune cell infiltration and immune regulatory genes in pan-cancer was analyzed by ESTIMATE and EPIC algorithms.The GO and KEGG enrichment analysis of TRIM5 related genes was further carried out,and the effect of TRIM5 on the proliferation and migration of pancreatic cancer was verified through in vitro experiments.Results TRIM5 was up-regulated in glioma,low-grade glioma,pancreatic cancer and other tumor tissues.The abnormal expression of TRIM5 was associated with poor prognosis in gliomas,low-grade brain gliomas,and pancreatic cancer,etc.TRIM5 promotes malignant progression of glioma,low-grade glioma of brain and pancreatic cancer.TRIM5 expression was associated with microsatellite instability,homologous recombination defects,tumor neoantigens,and tumor mutation burden.The expression of TRIM5 was positively correlated with the infiltration of various immune cells in pan cancer,such as T cells,macrophages,and cancer-related fibroblasts.TRIM5 was significantly correlated with immune regulatory genes in pan cancer,with most of them positively correlated with immune checkpoint genes such as PDCD1 and CTLA4.Gene enrichment results show that TRIM5 was closely related to signaling pathways such as cell adhesion and cancer-related signals.The in vitro experimental results showed that knocking down the TRIM5 gene significantly reduced the proliferation and migration ability of PANC-1 cells.Conclusion TRIM5 is abnormally expressed in various tumor tissues,and its expression is closely related to patient prognosis,clinical pathological characteristics,tumor immune infiltration,immune regulatory genes,genomic heterogeneity,etc.These results suggest that TRIM5 may be a new and promising tumor treatment target and prognostic biomarker.关键词
TRIM5/预后/免疫微环境/胰腺癌Key words
TRIM5/prognosis/immune micro-environment/pancreatic cancer分类
医药卫生引用本文复制引用
李伟凡,黄松音..TRIM5与恶性肿瘤不良预后相关性及其在胰腺癌恶性表型中的作用研究[J].岭南现代临床外科,2024,24(5):263-275,13.基金项目
国家自然科学基金(82071860) （82071860）
