茉莉酸和乙烯对巴西橡胶树橡胶生物合成的拮抗作用OA北大核心
Antagonistic Effects of Jasmonic Acid and Ethylene on Rubber Biosynthesis in Hevea brasiliensis
基于乙烯利(一种乙烯释放剂)刺激采胶可以显著增加每刀次的胶乳产量,以往认为橡胶生物合成主要受乙烯正调控.基于茉莉酸信号在促进植物次生代谢物质的生物合成中所起的关键作用,茉莉酸信号也被认为在天然橡胶生物合成的正反馈调节中起作用.最近的研究表明,割胶促进橡胶树合成天然橡胶与激活乳管细胞的茉莉酸信号途径密切相关.然而,茉莉酸信号途径和乙烯信号途径在橡胶生物合成调控过程中是否存在交互作用仍然不清楚.为此,本研究用茉莉酸甲酯和乙烯处理短期停割树,以 13C-MVA为底物测定全胶乳和小橡胶粒子的体外橡胶生物合成效率,用 qPCR 技术分析茉莉酸甲酯和乙烯处理条件下 4 个茉莉酸信号途径关键环节基因 HbCOI1、HbJ AZ1、HbMYC1、HbMYC2,4 个乙烯-响应元件结合因子(ERFs)基因HbERFⅢa、HbERFⅦa、HbERFⅨc、HbERFⅩa,4 个橡胶生物合成关键蛋白基因HbHMGR1、HbSRPP1、HbREF1、HbHRT2在萌条乳管细胞中的表达.结果表明:外源茉莉酸甲酯处理显著上调橡胶树乳管细胞中茉莉酸信号途径关键环节基因HbCOI1、HbJ AZ1、HbMYC1、HbMYC2和橡胶生物合成关键蛋白基因HbHMGR1、HbSRPP1、HbREF1、HbHRT2的表达水平,促进橡胶生物合成,但对乙烯-响应元件结合因子基因HbERFⅢa、HbERFⅦa、HbERFⅨc、HbERFⅩa的表达影响甚微,甚至抑制它们的表达.相反,外源乙烯处理显著上调橡胶树乳管细胞中乙烯-响应元件结合因子基因HbERFⅢa、HbERFⅦa、HbERFⅨc、HbERFⅩa的表达水平,但对茉莉酸信号途径关键环节基因HbCOI1、HbJ AZ1、HbMYC1、HbMYC2和橡胶生物合成关键蛋白基因HbHMGR1、HbSRPP1、HbREF1、HbHRT2的表达影响甚微,甚至抑制它们的表达,抑制橡胶生物合成.结果证明了茉莉酸和乙烯对巴西橡胶树橡胶生物合成的拮抗作用,将为阐明茉莉酸信号和乙烯信号的增产机理供理论依据.
Based on the fact that ethephon(an ethylene releaser)stimulation can significantly increase latex yield per tapping,it is traditionally believed that rubber biosynthesis is positively regulated by ethylene.Based on the critical role of jasmonic acid signaling in promoting the biosynthesis of secondary metabolites in plants,jasmonic acid signaling is also thought to play a role in the positive feedback regulation of natural rubber biosynthesis.Recent studies suggest that tapping-enhanced rubber biosynthesis is closely related to the activation of jasmonate signaling in laticifer cells of rub-ber tree.However,it remains unclear whether there is a crosstalk between the jasmonic acid signaling pathway and the ethylene signaling pathway in the regulation of rubber biosynthesis.For this purpose,in the present study,in vitro rub-ber biosynthesis efficiency of whole latex and small rubber particles(SRPs)from short rested trees treated by either methy jasmonates or ethylene were detected by using substrate 13C-MVA.qPCR was used to analyze the expression of four jasmonic acid signaling genes,HbCOI1,HbJ AZ1,HbMYC1,HbMYC2,four ethylene-responsive element binding factors(ERFs)genes HbERFⅢa,HbERFⅦa,HbERFⅨc,HbERFⅩa,four rubber biosynthesis genes,HbHMGR1,HbSRPP1,HbREF1,HbHRT2 in laticifer cells after epicormic shoots were treated with either methy jasmonates or eth-ylene.The results showed that exogenous methyl jasmonate treatment significantly up-regulated the expression levels of jasmonic acid signaling genes HbCOI1,HbJ AZ1,HbMYC1,HbMYC2,and rubber biosynthetic protein genes HbHMGR1,HbSRPP1,HbREF1,HbHRT2 in laticifer cells of rubber tree,and promoted rubber biosynthesis,but had little effect on the expression of ethylene-responsive element binding factor genes HbERFⅢa,HbERFⅦa,HbERFⅨc,HbERFⅩa,or even inhibited the expression.On the contrary,exogenous ethylene treatment significantly up-regulated the expression levels of ethylene-responsive element binding factor genes HbERFⅢa,HbERFⅦa,HbERFⅨc,HbERFⅩa in laticifer cells of rubber tree,but had little effect on the expression of jasmonic acid signaling genes HbCOI1,HbJ AZ1,HbMYC1,HbMYC2 and rubber biosynthetic protein genes HbHMGR1,HbSRPP1,HbREF1,HbHRT2 or even inhibited the ex-pression and rubber biosynthesis.The results suggest that there exists an antagonistic effects of jasmonic acid and eth-ylene on rubber biosynthesis in H.brasiliensis,which would provide a theoretical basis for elucidating the crosstalk of jasmonic acid signaling and ethylene signaling on regulation of natural rubber production.
杨署光;晁金泉;李言;吴绍华;张世鑫;邓小敏;史敏晶;田维敏
中国热带农业科学院橡胶研究所/农业农村部橡胶树生物学与遗传资源利用重点实验室/省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室,海南海口 571101中国热带农业科学院橡胶研究所/农业农村部橡胶树生物学与遗传资源利用重点实验室/省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室,海南海口 571101||中国热带农业科学院三亚研究院,海南三亚 572000||热带作物生物育种全国重点实验室,海南三亚 572000中国科学院西双版纳热带植物园,云南西双版纳 666303
农业科学
巴西橡胶树茉莉酸甲酯乙烯生物合成拮抗作用
Hevea brasiliensis Muell.Arg.methy jasmonatesethylenebiosynthesisantagonism
《热带作物学报》 2025 (001)
1-9 / 9
海南省自然科学基金项目(No.321MS0806);云南省重大科技专项计划项目(No.202402AE09001901);海南省重点研发计划项目(No.ZDYF2022XDNY252).
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