热带作物学报2025，Vol.46Issue(1)：10-20,11.DOI:10.3969/j.issn.1000-2561.2025.01.002
油茶KAS基因家族的鉴定及表达分析
Identification and Expression Profile Analysis of KAS Gene Family in Camellia oleifera
摘要
Abstract
Beta-ketoacyl-acyl carrier protein synthase(KAS)is the key enzyme system in plant fatty acid biosynthesis.In order to investigate the function of the KAS gene family in the maturation and oil accumulation of Camellia oleifera,this study used bioinformatics methods and analyzed ther physicochemical properties,chromosomal localization,sub-cellular localization,secondary structure,phylogenetic trees,conserved motifs,promoter cis-acting elements,and RT-qPCR to analyze the expression patterns in the oil rapid accumulation stage of C.oleifera.The findings indicated that 14 Camellia oleifera KAS family members were distributed on seven chromosomes with relative molecular masses between 1.13 kDa and 5.15 kDa,and most of them were acid-stabilized proteins.Except for CoKAS II-2,which was localized in mitochondria and chloroplasts,all of them were localized in chloroplasts.Phylogenetic analysis revealed that CoKAS was divided into three subclasses,with similarities in structure and conserved motifs among KAS family numbers in the same subfamily.The promoter regions of the CoKAS gene family members were enriched with the cis-acting ele-ments growth and development-related,light response,hormone induction,and adversity response.Expression analysis revealed that most of the CoKAS genes were highly expressed during the oil rapid accumulation stage.In addition,it was found a strong correlation between the members of the CoKAS and CoMYB gene families during fruit ripening in C.oleifera.The findings would provide a theoretical basis for comprehensively analyzing the function of CoKAS genes.关键词
油茶/CoKAS基因家族/生物信息学/表达分析Key words
Camellia oleifera/CoKAS gene family/bioinformatics/expression analysis分类
林学引用本文复制引用
刘晓霞,喻子昊,敬辉扬,熊凯峰,覃佐东..油茶KAS基因家族的鉴定及表达分析[J].热带作物学报,2025,46(1):10-20,11.基金项目
湖南省自然科学基金项目(No.2024JJ7188) （No.2024JJ7188）
湖南省教育厅科学研究项目(No.23C0354) （No.23C0354）
湖南科技学院科学研究项目(No.23XKY07). （No.23XKY07）
