热带作物学报2025，Vol.46Issue(1)：152-161,10.DOI:10.3969/j.issn.1000-2561.2025.01.016
杂交兰双艺金龙根状茎增殖、分化及生根壮苗研究
Rhizome Proliferation,Differentiation and Strong Seedlings of Cym-bidium‘Shuangyi Jinlong'
摘要
Abstract
Cymbidium‘Shuangyi Jinlong'is a new variety selected and bred from the clonal variation of the hybrid or-chid cultivar‘Golden dragon'by Fujian Bainong Ecological Technology Co.Ltd..It integrates flower and leaf art,has good germination,easy flowering and strong resistance,and has a high market demand.The rhizome and tissue culture seedlings of Cymbidium‘Shuangyi Jinlong'were used as experimental materials,and the orthogonal experiment was conducted to optimize the influencing factors of rhizome proliferation and differentiation,rooting and seedling growth,seedling cultivation and transplanting,in order to improve the seedling reproductive efficiency and seedling quality of hybrid orchid,and establish a standardized production system.The most suitable medium for rhizome proliferation and growth was MS medium supplemented with 1.00 mg/L NAA,0.10 mg/L 6-BA,20.00 g/L sucrose,1.00 g/L activated carbon,6.30 g/L agar,and 1.00 g/L peptone;the quantity value-added coefficient was determined to be 3.57 while the quality value-added coefficient was found to be 4.63.The optimal medium for rhizome differentiation was 1/2MS,1.50 mg/L NAA,0.90 mg/L TDZ,100.00 g/L banana homogenate,25.00 g/L sucrose 6.30g/L;the leaf bud differentia-tion rate reached up to 97.78%,with a differentiation coefficient of 6.87.The best-performing medium for rooting and seedlings strengthening was 1/2MS,l.00 mg/L IBA,100.00 g/L banana homogenate,0.17 g/L KH2PO4,30.00 g/L su-crose,agar and l.00 g/L activated carbon.After 90 days of cultivation,the average number of roots per plant was five,with a root length of 5.40 cm,a pseudobulb thickness of 4.35 mm,a plant height of 12.95 cm,and a fresh quality of 2.42 g.The recommended method for transplanting tissue culture seedlings involves using water hyacinth∶pine bark(V∶V=1∶1)as the transplantation substrate after 21 days in vitro culture.The survival rate after 60 days post-transplantation reached 83.33%.Taking into account the impact of various factors,this study ultimately identified the optimal medium compo-sitions at each stage and refined transplanting schemes.This provides a reference for establishing a standardized pro-duction system.关键词
杂交兰/根状茎/增殖/分化/生根壮苗Key words
Cymbidium hybrid/rhizome/proliferation/differentiation/rooting and seedlings strengthening分类
农业科技引用本文复制引用
邓淑雯,翟俊文,吴悠,张华园,李世星,林子润,陈南川,傅春梅,饶小琼,吴沙沙..杂交兰双艺金龙根状茎增殖、分化及生根壮苗研究[J].热带作物学报,2025,46(1):152-161,10.基金项目
国家重点研发计划项目(No.2020YFD1000400) （No.2020YFD1000400）
福建农林大学植物新资源开发与利用服务团队项目(No.11899170124) （No.11899170124）
福建农林大学2021年度乡村振兴服务团队项目(No.11899170151). （No.11899170151）
