热带作物学报2025，Vol.46Issue(1)：171-179,9.DOI:10.3969/j.issn.1000-2561.2025.01.018
短毛木耳的生物学特性与驯化栽培研究
Biological Characteristics and Acclimation Cultivation of Auricularia villosula
摘要
Abstract
Auricularia villosula Malysheva is a similar species of Auricularia heimuer F.Wu,B.K.Cui&Y.C.Dai,mainly distributed in high altitude areas of tropical and subtropical regions.It can adapt to the high temperature and high humidity climate in Guangxi.There are few reports on the domestication of A.villosula.In this paper,the fruiting bod-ies of auriculae collected in the field was used,and the molecular marker was established by sequence analysis.The effects of different temperature,carbon and nitrogen sources on the mycelia growth of A.villosula were explored through single factor tests,and the carbon and nitrogen sources of the medium were further optimized by an orthogonal test.The fruiting body of fungus was preliminarily identified as A.villosula(TY081).The results showed that the de-veloped molecular labeling method could quickly and effectively identify A.villosula.The optimal temperature for my-celia growth of A.villosula TY081 was 30℃,the optimal carbon source was glucose,and the optimal nitrogen source was soybean powder.The optimized medium carbon and nitrogen combination was fructose,yeast powder,mycelium growth rate reached 0.92 mm/d.A.villosula TY081 was incubated at 28℃in a culture bag mainly composed of saw-dust,and the bag was full for 60 days.After puncturing,it was cultured in a greenhouse for 10 days to form the primor-dia.The fruity body growth temperature was 24℃,the humidity was 85%,and the yield of each bag was 20.943 g(dry weight).关键词
短毛木耳/分子验证/生物学特性/驯化栽培Key words
Auricularia villosula/molecular verification/biological characteristics/acclimation culture分类
农业科学引用本文复制引用
聂晨红,周炳沼,丰景,韦仕岩,祁亮亮,王晓国..短毛木耳的生物学特性与驯化栽培研究[J].热带作物学报,2025,46(1):171-179,9.基金项目
广西农科院基本业务费专项(桂农科2021YT094,桂农科2025YP075) （桂农科2021YT094,桂农科2025YP075）
国家现代农业产业技术体系建设项目(No.CARS-20). （No.CARS-20）
