CYP2亚家族成员在结肠癌中的表达水平及功能验证研究OACSTPCD

Objective:To screen the differentially expressed CYP2 family genes in colon carcinoma through public data-bases and cell functional experiments,and systematically analyze the expression and functions of CYP2 family genes in colon carcinoma.Methods:The Cancer Genome Atlas database was used to identify the differentially expressed genes(DEGs)in CYP2 subfamily in colon carcinoma.The R package was run for prognosis and gene function enrichment analyses.EdU was applied to detect the effect of overexpression of CYP2J2 on cell proliferation in colon carcinoma,and scratch healing and tran-swell chamber assays were applied to detect the effect of over-expression of CYP2J2 on cell migration in colon carcinoma.Results:CYP2J2,CYP2C18,CYP2U1 and CYP2D6 were down-regulated(P＜0.01)in colon carcinoma,whereas CYP2S1 and CYP2W1 were up-regulated(P＜0.01).The lower expres-sions of CYP2J2 and CYP2C18 were related to worse prognosis of colon carcinoma patients(P＜0.01).Decreased expres-sions of CYP2J2 and CYP2C18 were both early events during the progression of colon carcinoma.KEGG results showed that CYP2J2 was related to signaling pathways such as extracellular matrix-receptor interaction and focal adhesion.Moreover,cy-tological experiments revealed that overexpression of CYP2J2 significantly inhibited the proliferation and migration abilities of colon carcinoma cells(P＜0.01).Conclusion:Low expressions of CYP2J2 and CYP2C18 were related to the worse progno-sis of colon carcinoma patients,and overexpression of CYP2J2 could inhibit the proliferation and migration abilities of colon carcinoma cells,suggesting that CYP2J2 might be a potential prognostic marker and therapeutic target of colon carcinoma.