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CYP2亚家族成员在结肠癌中的表达水平及功能验证研究

蒋琴 周航宇 魏远 王宏

肿瘤预防与治疗2024，Vol.37Issue(12)：1015-1023,9.
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肿瘤预防与治疗2024，Vol.37Issue(12)：1015-1023,9.DOI:10.3969/j.issn.1674-0904.2024.12.001

CYP2亚家族成员在结肠癌中的表达水平及功能验证研究

Expression Levels and Functional Verification of Members of the CYP2 Subfamily in Colon Cancer

蒋琴 1周航宇 2魏远 3王宏4

作者信息

  • 1. 621000 四川绵阳,四川中医药高等专科学校基础医学部人体解剖学与组织胚胎学教研室
  • 2. 621000 四川绵阳,绵阳市第一人民医院/绵阳四○四医院普外科
  • 3. 621000 四川绵阳,绵阳市第一人民医院/绵阳四○四医院医学影像科
  • 4. 621000 四川绵阳,绵阳市第一人民医院/绵阳四○四医院介入放射科
  • 折叠

摘要

Abstract

Objective:To screen the differentially expressed CYP2 family genes in colon carcinoma through public data-bases and cell functional experiments,and systematically analyze the expression and functions of CYP2 family genes in colon carcinoma.Methods:The Cancer Genome Atlas database was used to identify the differentially expressed genes(DEGs)in CYP2 subfamily in colon carcinoma.The R package was run for prognosis and gene function enrichment analyses.EdU was applied to detect the effect of overexpression of CYP2J2 on cell proliferation in colon carcinoma,and scratch healing and tran-swell chamber assays were applied to detect the effect of over-expression of CYP2J2 on cell migration in colon carcinoma.Results:CYP2J2,CYP2C18,CYP2U1 and CYP2D6 were down-regulated(P＜0.01)in colon carcinoma,whereas CYP2S1 and CYP2W1 were up-regulated(P＜0.01).The lower expres-sions of CYP2J2 and CYP2C18 were related to worse prognosis of colon carcinoma patients(P＜0.01).Decreased expres-sions of CYP2J2 and CYP2C18 were both early events during the progression of colon carcinoma.KEGG results showed that CYP2J2 was related to signaling pathways such as extracellular matrix-receptor interaction and focal adhesion.Moreover,cy-tological experiments revealed that overexpression of CYP2J2 significantly inhibited the proliferation and migration abilities of colon carcinoma cells(P＜0.01).Conclusion:Low expressions of CYP2J2 and CYP2C18 were related to the worse progno-sis of colon carcinoma patients,and overexpression of CYP2J2 could inhibit the proliferation and migration abilities of colon carcinoma cells,suggesting that CYP2J2 might be a potential prognostic marker and therapeutic target of colon carcinoma.

关键词

CYP2J2/结肠癌/公共数据/细胞迁移

Key words

Colon carcinoma/CYP2J2/Public data/Cell migration

分类

医药卫生

引用本文复制引用

蒋琴,周航宇,魏远,王宏..CYP2亚家族成员在结肠癌中的表达水平及功能验证研究[J].肿瘤预防与治疗,2024,37(12):1015-1023,9.

基金项目

This study was supported by grants from Health Commission of Mianyang(No.202205). 绵阳市卫健委2022年科研课题补助项目(编号:202205) （No.202205）

肿瘤预防与治疗

OACSTPCD

1674-0904

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