生态环境学报2024，Vol.33Issue(12)：1931-1943,13.DOI:10.16258/j.cnki.1674-5906.2024.12.011
伴矿景天对重金属-多环芳烃复合污染土壤有机污染物消减及微生物的影响
Influence of Sedum plumbizincicola on the Reduction of Organic Contaminants and Microorganisms in Soil Contaminated with Heavy Metals and Polycyclic Aromatic Hydrocarbons
摘要
Abstract
The widespread issue of soil co-contamination with heavy metals and organic compounds presents a significant challenge to environmental remediation.Metal-hyperaccumulating plants can remove heavy metals from soil by absorbing and accumulating them in their tissues.However,their effects on the removal of organic pollutants in co-polluted soils,particularly the role of rhizosphere microorganisms in this process,remain unclear This study investigated the influence and mechanism of Sedum plumbizincicola on the bioremediation of phenanthrene(PHE)in cadmium(Cd)-PHE co-contaminated soils under different Cd concentrations.Analysis of the PHE degradation rates,microbial community,and abundance of PHE-degrading bacteria at different Cd levels in the soil revealed that Cd inhibited PHE degradation.The PHE degradation rates in treatments with and without S.plumbizincicola were reduced by 25.6%and 13.3%,respectively,when a Cd concentration of 10 mg·kg-1 was added.However,S.plumbizincicola significantly promoted PHE degradation in Cd-PHE co-contaminated soil at lower Cd concentrations,and increased the abundance of potential PHE-degrading bacteria in the rhizosphere soil.The average degradation rate and abundance of potential degraders were 6.77%and 0.538%,respectively,which were higher than those in the unplanted treatment.Further analysis coupled with the potential functional microbes identified by stable isotope probing(SIP)indicated that Cd inhibited the activity of certain microorganisms,such as those represented by Micrococcaceae,which are intolerant or have low Cd tolerance,thereby hindering PHE degradation in the soil.Conversely,S.plumbizincicola alleviated the toxicity of Cd to potential PHE-degrading bacteria and enriched specific PHE-degrading bacteria,such as Micrococcaceae and Ellin6067,which are plant growth-promoting bacteria in the rhizosphere.This enrichment enhanced the total abundance of potential PHE-degrading bacteria and facilitated PHE degradation in soil.Additionally,Ellin6067,KD3-93,Flavisolibacter,and Adhaeribacter have been identified as potential PHE degraders using DNA-SIP.This study provides theoretical guidance for the application of metal-tolerant or-hyperaccumulating plants in the remediation of soils co-contaminated with heavy metals and polycyclic aromatic hydrocarbons.关键词
镉/菲/复合污染/伴矿景天/微生物群落/稳定同位素探针Key words
cadmium/phenanthrene/co-contamination/Sedum plumbizincicola/microbial community/stable isotope probing分类
天文与地球科学引用本文复制引用
官国庆,黄紫琳,江龙飞,罗春玲..伴矿景天对重金属-多环芳烃复合污染土壤有机污染物消减及微生物的影响[J].生态环境学报,2024,33(12):1931-1943,13.基金项目
国家自然科学基金国际(地区)合作与交流项目(32061133003) （地区）
