应用化学2024，Vol.41Issue(12)：1760-1769,10.DOI:10.19894/j.issn.1000-0518.240055
稀土荧光探针用于快速检测乙肝表面抗原
Rare Earth Fluorescent Probes for Rapid Detection of Hepatitis B Surface Antigen
摘要
Abstract
In this study,a self-synthesized rare-earth chelator 4,4′-Bis(1″,1″,1″,2″,2″,3″,3″-7-fluoro-4″,6″-hexanedione-6″-yl)chlorosulfonated terphenyl (BHHCT) was used to prepare hepatitis B surface antibody markers,and a time-resolved fluorescence immunoassay for the rapid detection of hepatitis B surface antigen (HBsAg) was developed by applying lateral-flow immunochromatographic test strips and an accompanying time-resolved fluorescence detection system. The linear range was 0.2～500 ng/mL,the detection limit was 0.16 ng/mL,and the linear regression coefficient of determination (R2) was 0.9996. The intra-batch coefficient of variation was less than 5％,the inter-batch coefficient of variation was less than 8％,and the recoveries of the samples ranged from 94.3％ to 107.8％. The method has the advantages of precision,rapidity,specificity and no need for specialized personnel to operate,which can meet the requirement of immediate detection of hepatitis B surface antigen.关键词
时间分辨荧光免疫分析法/侧流免疫层析试纸条/乙肝表面抗原/即时检测Key words
Time-resolved fluorescence immunoassay/Lateral flow immunochromatography strip/Hepatitis B surface antigen/Point of care testing分类
化学化工引用本文复制引用
张瑞起,常宇,刘作家,李雪松,潘利华..稀土荧光探针用于快速检测乙肝表面抗原[J].应用化学,2024,41(12):1760-1769,10.基金项目
新疆维吾尔自治区科技厅重点研发项目(No.2021B03003)、吉林省科技厅重点研发项目(No.20224094094XY)和吉林省自然科学基金面上项目(No.20230101330JC)资助Supported by Xinjiang Uygur Autonomous Region Science and Technology Department Key Research and Development Project(No.2021B03003),Jilin Provincial Science and Technology Department Key Research and Development Project(No.20224094094XY)and Jilin Provincial Natural Science Foundation Upper-level Project(No.20230101330JC) （No.2021B03003）
