应用化学2024，Vol.41Issue(12)：1770-1779,10.DOI:10.19894/j.issn.1000-0518.240179
苯二氮卓类毒品波玛唑仑的体内外代谢产物表征
Investigation on in vivo and in vitro Metabolites of Bromazolam
摘要
Abstract
The abuse of new psychoactive substances (NPS) shows a significant growth trend around the world,which has the characteristics of a wide variety,a wide epidemic range and the strong addiction. Bromazolam,which appears in the illegal market as a benzodiazepine NPS,is usually used to enhance the stimulating effect of opioids or to use its hypnotic and amnesia side effects to carry out drug sexual assault. In this study,the metabolic model of bromazolam in zebrafish and human liver microsomes was established. Phase Ⅰ and Ⅱ metabolites of bromazolam were identified by liquid chromatography-high resolution mass spectrometry (LC-HRMS). Seven metabolites were identified,of which six were phase Ⅰ metabolites and one was a phase Ⅱ metabolite. The metabolic pathways of bromazolam include monohydroxylation (M1),dihydroxylation (M2),monohydroxylation+reduction (M3),dealkylation (M4),oxidative deamination (M5),monohydroxylation+oxidative deamination (M6) and monohydroxylation+glucuronidation (M7). Monohydroxylation metabolites (M1) are recommended as the possible biomarker of bromazolam. In this paper,the metabolism of bromazolam in vivo was studied for the first time,and the information of metabolites and metabolic pathways of bromazolam was improved,which is helpful to provide methods and data for its abuse.关键词
新精神活性物质/波玛唑仑/代谢/斑马鱼/肝微粒体Key words
New psychoactive substances/Bromazolam/Metabolism/Zebrafish/Human liver microsomes分类
化学化工引用本文复制引用
汤以绫,赵君博,张淼,钱小雨,向平,严慧..苯二氮卓类毒品波玛唑仑的体内外代谢产物表征[J].应用化学,2024,41(12):1770-1779,10.基金项目
国家重点研发计划(No.2022YFC3302003)、中央公益课题(No.GY2022G-2)、上海市法医学重点实验室资助项目(No.21DZ2270800)、司法部司法鉴定重点实验室资助项目和上海市司法鉴定专业技术服务平台资助项目(No.19DZ2292700)资助Supported by the National Key Research and Development Program of China(No.2022YFC3302003),the Ministry of Finance,PR China(No.GY2022G-2),Shanghai Key Laboratory of Forensic Science(No.21DZ2270800),the Key Laboratory of Forensic Expertise of the Ministry of Justice and Shanghai Forensic Service Platform(No.19DZ2292700) （No.2022YFC3302003）
