现代畜牧科技Issue(12)：23-25,3.DOI:10.19369/j.cnki.2095-9737.2024.12.007
不同标记二抗在免疫组化检测CD3和CD21的比较研究
Comparative Study of Different Labeled Secondary Antibodies in Immunohistochemical Detection of CD3 and CD21
摘要
Abstract
Immunohistochemistry technology plays an important role in the diagnosis and treatment of animal diseases.In this experiment,paraffin-embedded sections of porcine mesenteric lymph nodes and mouse spleen were used to investigate the expression of CD3 and CD21 proteins using three different secondary antibodies(HRP,Polymer HRP and AIexa FIuorTM 488).The results showed that after immunohistochemical staining with three different labeled secondary antibodies,CD3 and CD21 positive signals could be displayed in lymph nodes and spleen.In the experimental groups using enzymes to label secondary antibodies,the average optical density value of the Polymer HRP group was significantly higher than that of the HRP group.And using AIexa FIuorTM 488 to label the secondary antibody in the experimental can also obtain higher positive signals.This indicates that the use of different labeled secondary antibodies in immunohistochemical detection has a certain impact on the detection results of tissue antigens.关键词
免疫/第二抗体/免疫酶法/免疫荧光法Key words
immune/second antibody/immunoenzyme method/immunofluorescence method分类
医药卫生引用本文复制引用
唐金宝,李佳露,樊含笑,孙启航,段少仪,胡湘翎,苏建明,雷红宇..不同标记二抗在免疫组化检测CD3和CD21的比较研究[J].现代畜牧科技,2024,(12):23-25,3.基金项目
湖南省自然科学基金项目(2022JJ30022,2022JJ30293) （2022JJ30022,2022JJ30293）
湖南省技术攻关"揭榜挂帅"项目(2021NK1030) （2021NK1030）
