中国病理生理杂志2024，Vol.40Issue(12)：2312-2318,7.DOI:10.3969/j.issn.1000-4718.2024.12.015
癸基泛醌对中波紫外线诱导的HaCaT细胞光损伤的作用及其机制
Effect of decylubiquinone on UVB-induced photodamage in HaCaT cells and its mechanism
摘要
Abstract
AIM:To investigate the protective effect of decylubiquinone(DUb)against medium-wave ultravio-let(UVB)-induced photodamage in HaCaT cells and its molecular mechanism.METHODS:The photodamage model was established by irradiating HaCaT cells with UVB.The experiment was divided into 6 groups:control group,UVB model group(30 mJ/cm2),UVB+low-,medium-and high-concentration(2.5,5 and 10 µmol/L)DUb groups,and DUb(10 µmol/L)group.Cell viability was detected using the CCK-8 assay.The morphological changes of the HaCaT cells were observed under a light microscope.The production of reactive oxygen species(ROS)content in the HaCaT cells was analyzed by fluorescence probe of DCFH-DA,and the superoxide dismutase(SOD)activity,intracellular glutathione(GSH)and malondialdehyde(MDA)levels were detected using biochemical reagents.The apoptosis rate was detected through flow cytometry.The protein expression levels of Bax、Bcl-2、caspase-3 and p53 were detected by Western blot.RESULTS:Compared with the control group,the cell viability of UVB model group was significantly decreased(P＜0.01),and obvious morphological changes,ROS and MDA were significantly increased(P＜0.01),GSH and SOD were significantly decreased(P＜0.01),the apoptosis rate was increased(P＜0.01).DUb pretreatment significantly increased the viability of UVB-induced photodamage HaCaT cells(P＜0.01),decreased intracellular ROS and MDA production(P＜0.05,P＜0.01),increased GSH content and SOD activity(P＜0.05,P＜0.01),and reduced apoptosis(P＜0.01).Addi-tionally,the protein expression levels of Bax,caspase-3 and p53 in medium-and high-dose DUb groups were significantly decreased(P＜0.01),while the anti-apoptotic protein Bcl-2 was significantly increased(P＜0.01).CONCLUSION:Dec-ylubiquinone can alleviate UVB-induced photodamage to HaCaT cells,reduce apoptosis and enhance the antioxidant ca-pacity of cells,and protect HaCaT cells against UVB radiation.关键词
癸基泛醌/中波紫外线/HaCaT细胞/氧化应激/细胞凋亡Key words
decylubiquinone/ultraviolet B/HaCaT cells/oxidative stress/apoptosis分类
医药卫生引用本文复制引用
王妍,赵波,童嘉玲,马宇昕,王欣,亓翠玲,唐佩..癸基泛醌对中波紫外线诱导的HaCaT细胞光损伤的作用及其机制[J].中国病理生理杂志,2024,40(12):2312-2318,7.基金项目
国家自然科学青年基金项目(No.82302827) （No.82302827）
广东省中医药局科研项目(No.20242048) （No.20242048）
广州市基础与应用基础项目(No.202201010299 （）
No.202201010307) （）
广州市基础与应用基础优秀博士"续航"项目(No.2025A04J5306) （No.2025A04J5306）
