中国乳业Issue(12)：104-108,5.DOI:10.12377/1671-4393.24.12.18
胶体金免疫层析法测定生乳中抗生素含量的不确定度
Uncertainty Evaluation of Antibiotic Content in Raw Milk Measured by Colloidal Gold Immunochromatography
陈炜楠 1杨爱君 1何瑛 1梁俊健 1曹学思1
作者信息
- 1. 广东燕塘乳业股份有限公司,广东广州 510630
- 折叠
摘要
Abstract
[Objective]To evaluate the uncertainty of the readout results of antibiotic content (chloramphenicol and fluoroquinolones) in raw milk using Colloidal Gold Immunochromatographic Assay.[Method]The "Yirui Chloramphenicol Single Test Rapid Detection Test Strip"[1]was used for chloramphenicol content measurement,and the "Yirui Fluoroquinolones Antibiotics Rapid Detection Test Strip in Milk"[2]was used for fluoroquinolones content measurement.By analyzing the sources of uncertainty,a mathematical model was constructed.Each part of the model was analyzed and evaluated,and the relative standard uncertainty was synthesized and further expanded to obtain the expanded uncertainty of antibiotic content (chloramphenicol and fluoroquinolones) in raw milk.[Result]At a confidence level of 95％,the readout results of antibiotic content in raw milk were:X(chloramphenicol)=3.20±0.0596,with k=2 and an expanded uncertainty U=0.0596;X(fluoroquinolones)=3.00±0.0594,with k=2 and an expanded uncertainty U=0.0594.[Conclusion]The uncertainty in the experimental process mainly originates from sample repeatability,sampling volume,test strip reader,and uncertainty introduced in result calculation.This method can provide a reference for uncertainty evaluation of antibiotic content in raw milk measured by immunoassay(colloidal gold immunochromatography).关键词
免疫分析法/生乳/胶体金免疫层析法/抗生素/不确定度Key words
immunoassay/raw milk/colloidal gold immunochromatography/antibiotics/uncertainty引用本文复制引用
陈炜楠,杨爱君,何瑛,梁俊健,曹学思..胶体金免疫层析法测定生乳中抗生素含量的不确定度[J].中国乳业,2024,(12):104-108,5.
