中国现代中药2024，Vol.26Issue(12)：2100-2105,6.DOI:10.13313/j.issn.1673-4890.20240319001
基于HPLC指纹图谱的紫花亚菊差异性研究
Differential Analysis of Ajania purpurea C.Shih Based on HPLC Fingerprint
摘要
Abstract
Objective:To establish a high-performance liquid chromatography(HPLC)fingerprint and multi-component content determination method for Ajania purpurea C.Shih.,and compare and analyze 19 batches of samples collected from different locations and times,as well as medicinal parts,to assess their differences.Methods:HPLC was used with 0.1%phosphoric acid solution and acetonitrile as the mobile phase,gradient elution,and a detection wavelength of 320 nm.A fingerprint was established.Based on similarity evaluation,combined with cluster analysis,principal component analysis(PCA),and orthogonal partial least squares discriminant analysis(OPLS-DA),the content of chlorogenic acid and isochlorogenic acid in A.purpurea was determined simultaneously.Results:The fingerprint spectra for the 19 batches of A.purpurea samples were established,with similarity ranging from 0.810 to 0.997.Fourteen common peaks were identified,with chlorogenic acid and isochlorogenic acid recognized as key components,which can serve as quality markers for evaluating A.purpurea from different sources and collection times,and medicinal parts.Cluster analysis and PCA of the 14 common peaks showed minimal component differences between different locations,but significant chemical differences between different collection times.Quantitative analysis revealed statistically significant differences in the chemical component content of A.purpurea medicinal parts and those from different locations,with higher levels of chlorogenic acid and isochlorogenic acid in samples collected in August.Conclusion:The establishment of the HPLC fingerprint and content determination method for A.purpurea is feasible and can provide a reference for the overall quality evaluation of A.purpurea medicinal materials.关键词
坎阿仲/紫花亚菊/高效液相色谱法/指纹图谱/含量测定/化学模式识别Key words
Kanazhong/Ajania purpurea C.Shih./HPLC/fingerprint/content determination/chemical pattern recognition分类
医药卫生引用本文复制引用
卓玛措毛,白玛次旦,久美,才让南加..基于HPLC指纹图谱的紫花亚菊差异性研究[J].中国现代中药,2024,26(12):2100-2105,6.基金项目
2021年西藏自治区藏医药管理局局级项目(JJKT0016) （JJKT0016）
