中国现代中药2024，Vol.26Issue(12)：2106-2114,9.DOI:10.13313/j.issn.1673-4890.20240529004
基于HPLC特征图谱和含量测定的红花标准汤剂研究
Study on Safflower Standard Decoction Based on HPLC Characteristic Chromatogram and Content Determination
摘要
Abstract
Objective:This paper aims to establish the HPLC characteristic chromatogram of safflower standard decoction and determine the content of kaempferol and 6-methoxykaempferol.Methods:15 batches of safflower standard decoction were prepared,and the extraction rate was determined.The chromatogram of standard decoction was established by high performance liquid chromatography,and common characteristic peaks of standard decoction were analyzed by mass spectrometry and compared with reference standards.The similarity was evaluated.The contents and transfer rate of kaempferol and 6-methoxykaempferol were also determined.Results:The extraction rate of 15 batches of safflower standard decoction ranges from 26.0%to 36.5%,with an average of 31.7%.There are 12 common characteristic peaks in the chromatogram,and seven components are identified,namely,syringin(peak 1),hydroxysafflor yellow A(peak 3),tryptophan(peak 4),kaempferol-3-O-sophoroside(peak 7),rutin(peak 8),kaempferol-3-O-rutin(peak 9),and bidenoside C(peak 12).The similarity is greater than 0.915.The mass fraction of kaempferol in standard decoction is 0.39-1.00 mg·g-1,and the transfer rate is 45.9%-78.9%.The mass fraction of 6-methoxykaempferol is 0.20-1.15 mg·g-1,and the transfer rate is 41.2%-89.8%.Conclusion:The chemical components of 15 batches of safflower decoction pieces and standard decoction can be transferred well and stably,and the established methods are simple and reliable,which can provide a reference for the establishment and evaluation of quality standards.关键词
红花/标准汤剂/山柰酚/6-甲氧基山柰酚/特征图谱Key words
safflower/standard decoction/kaempferiol/6-methoxykaempferol/chromatogram分类
医药卫生引用本文复制引用
魏家保,唐双燕,谢嘉慧,胡雨,张辉,谭沛..基于HPLC特征图谱和含量测定的红花标准汤剂研究[J].中国现代中药,2024,26(12):2106-2114,9.基金项目
深圳市科技计划项目(JSGG20191129093418578) （JSGG20191129093418578）
淮北市科技计划项目(KH2022AB007) （KH2022AB007）
