中国中西医结合杂志2024，Vol.44Issue(12)：1482-1487,6.DOI:10.7661/j.cjim.20240531.114
强精片对初老雄性大鼠精子质量及雄激素生成、利用的影响
Effects of Qiangjing Tablets on Sperm Quality,Androgen Production and Utilization in Pre-Ageing Male Rats
摘要
Abstract
Objective To observe the effects of Qiangjing Tablets(QJT)on sperm quality and the production and utilization of androgens in pre-ageing male rats,and to explore the possible mechanisms through the androgen receptor(AR)and testosterone synthesis pathways.Methods Six 2-month-old SD rats were set as the control group,and 24 19-month-old SD rats were randomly divided into model group,QJT low,medium and high dose group,with 6 rats in each group.The control group and the model group were treated with normal saline by gavage,and the QJT low,medium and high dose group were treated with corresponding doses of QJT(0.72,1.44,2.88 g·kg-1·d-1,respectively).The intervention lasted for 4 weeks.Sperm density,sperm viability and motile sperm rate were measured.Serum luteinizing hormone(LH),total testosterone(TT),free testosterone(FT),and sex hormone-binding globulin(SHBG)were measured by ELISA,and testosterone secretion index(TSI)was calculated.Protein expression of testis AR,steroid acute production regulatory protein(StAR),cytochrome P450 side chain lyase(CYP11A1),cytochrome P450 17-α-hydroxylase/20-lyase(CYP17A1)and 3 β-hydroxy steroid dehydrogenase(3β-HSD)were detected by Western Blot.Results Compared with the control group,the sperm density,sperm viability,sperm motility rate and TSI decreased(P＜0.01),while serum SHBG and the expression of testis AR,StAR and 3 β-HSD increased(P＜0.05,P＜0.01)in the model group.Compared with the model group,the sperm density,sperm viability and motile sperm rate increased in QJT high dose group(P＜0.01).TSI decreased(P＜0.05),expression of testis CYP17AR increased in QJT high and medium dose group(P＜0.01).The expression of testis AR increased(P＜0.01),the expression of testis StAR,3β-HSD and CYP11A1 protein decreased(P＜0.01)in the QJT low,medium,and high dose group.Conclusion QJT could improve sperm quality in pre-ageing male rats,alleviate the compensation of testosterone production and increase the utilization of androgen in the testis of pre-ageing male rats,which may be related to the regulation of StAR,3β-HSD,CYP11A1 and CYP17A1 and the up-regulation of AR expression in the testicular tissue.关键词
强精片/初老/生殖衰老/精子/雄激素/中药复方Key words
Qiangjing Tablets/pre-ageing/reproductive senescence/sperm/androgen/Chinese herbal compound引用本文复制引用
严旭洪,常德贵,陈帝昂,尤耀东,李广森,张培海,黄晓朋,董良,申毅锋,俞旭君..强精片对初老雄性大鼠精子质量及雄激素生成、利用的影响[J].中国中西医结合杂志,2024,44(12):1482-1487,6.基金项目
国家自然科学基金面上项目(No.81973647) （No.81973647）
国家卫生健康委男性生殖健康重点实验室开放课题面上项目(No.2021GJP03) （No.2021GJP03）
成都中医药大学"杏林学者"学科人才科研提升计划"优秀人才"项目(No.YXRC2020001) （No.YXRC2020001）
