中国中西医结合杂志2024，Vol.44Issue(12)：1488-1495,8.DOI:10.7661/j.cjim.20241113.245
益元灸调控TRPV1/C-kit/HCN1改善脊髓损伤后神经源性膀胱大鼠尿动力学
Yiyuan Moxibustion Improves Urodynamics of Neurogenic Bladder Rats after Spinal Cord Injury Based on Regulating TRPV1/C-kit/HCN1
摘要
Abstract
Objective To observe the effect of Yiyuan moxibustion(YM)on urodynamics of neurogenic bladder(NB)rats after sacral spinal cord injury(SCI),and to explore its mechanism based on transient receptor potential vanillic acid 1(TRPV1),C-kit and hyperpolarization-activated cyclic nucleotide cation channel 1(HCN1)pathway.Methods Totally 72 SD rats were randomly divided into sham operation group(n=12),model group(n=15),YM group(n=15),inhibitor group(n=15)and YM+inhibitor group(n=15).The rat model of NB after SCI was established by modified Hassan Shaker spinal cord transection method.The sham operation group only exposed the spinal cord and did not transect.Except sham operation group,each group was given daily manual urination.The YM group was treated with YM at Shenque(CV8)and Guanyuan(CV4).The inhibitor group was given TRPV1 inhibitor capsazepine(2.5 mL,100 nmol/L,30 min)for bladder perfusion.The YM+inhibitor group was given YM and capsazepine intervention,the intervention course for all was 14 consecutive days.The bladder function of rats was detected by urodynamics,the morphological changes of bladder tissue were observed by HE staining,and the ATP content in bladder tissue was detected by colorimetry.The positive expression of interstitial cells of Cajal(ICC)and HCN1 in bladder tissue were observed by immunofluorescence double staining.The protein and mRNA expressions of TRPV1,C-Kit and HCN1 in bladder tissues were detected by Western Blot and RT-PCR,respectively.Results The bladder tissue structure of rats in the sham operation group was complete and orderly.The bladder epithelial cells in the model group and inhibitor group were disordered,the lamina propria was damaged,the muscle bundles were blurred,the fibers were contracted,the tissue edema and hemorrhage were observed.YM group and the YM+inhibitor group were more regular in the arrangement of bladder tissue structure,the vacuolar cells were decreased,the muscle fibers were integrity,and the tissue edema and bleeding were relief compared with model group and inhibitor group,respectively.Compared with the sham operation group,the maximum bladder capacity and bladder compliance increased(P＜0.01),and the leak point pressure,ATP content in bladder tissue,C-Kit,HCN1 receptor fluorescence intensity,TRPV1,C-Kit,HCN1 protein and mRNA expression decreased in model group(P＜0.01).Compared with the model group and inhibitor group,the maximum bladder capacity and bladder compliance decreased(P＜0.01),and the leak point pressure,ATP content in bladder tissue,C-Kit,HCN1 receptor fluorescence intensity,TRPV1,C-Kit,HCN1 protein and mRNA expression increased in YM group and YM+inhibitor group(P＜0.05,P＜0.01),respectively.The maximum bladder capacity and bladder compliance increased(P＜0.01),the leak point pressure,ATP content in bladder tissue,C-Kit,HCN1 receptor fluorescence,TRPV1,C-Kit,HCN1 protein and mRNA expression decreased in inhibitor group than model group(P＜0.05,P＜0.01).Conclusions YM can improve the urodynamics of NB rats after SCI,and its mechanism may related to the regulation of TRPV1/C-ki/HCN1 pathway in bladder tissue.关键词
脊髓损伤/神经源性膀胱/尿流动力学/瞬时受体电位香草酸1/Cajal间质细胞/超极化激活环核苷酸阳离子通道/益元灸/艾灸/中西医结合Key words
spinal cord injury/neurogenic bladder/urodynamics/instantaneous receptor potential vanillic acid 1/cajal interstitial cells/hyperpolarization activates cyclic nucleotide cation channels/Yiyuan moxibustion/moxibustion/integrative medicine引用本文复制引用
黄晓萌,张芝兰,韦慧麟,李冰,尚文雅,黄靖,任亚锋..益元灸调控TRPV1/C-kit/HCN1改善脊髓损伤后神经源性膀胱大鼠尿动力学[J].中国中西医结合杂志,2024,44(12):1488-1495,8.基金项目
河南省中医药科学研究专项课题(No.2022JDZX015,No.2021 JDZY022) （No.2022JDZX015,No.2021 JDZY022）
