草业学报2025，Vol.34Issue(1)：174-190,17.DOI:10.11686/cyxb2024133
紫花苜蓿GLK基因家族鉴定及渗透胁迫下的表达分析
Genome-wide identification of the GLK gene family in alfalfa and their transcript profiles under osmotic stress
摘要
Abstract
GLK(Golden2-like or G2-like)transcription factors belong to the GARP superfamily,whose members play crucial roles in plant growth and development.Members of the GLK gene family play important roles in regulating plant chloroplast development,chlorophyll biosynthesis,and the abiotic stress response.The GLK gene family has been systematically identified in many plant species,but not yet in tetraploid alfalfa(Medicago sativa).In this study,we used bioinformatics methods to identify 100 GLK genes(MsGLKs)in the genome of the alfalfa cultivar"Xinjiang Daye".Further analyses were conducted to explore the physicochemical properties of their putative encoded products,chromosome localization,phylogenetic relationships,cis-acting elements in their promoter regions,and transcript profiles under osmotic stress and exogenous abscisic acid(ABA)treatment.The results showed that the 100 MsGLK genes were unevenly distributed on the 32 chromosomes,and encoded polypeptides with sequence lengths ranging from 201 to 860 amino acids.In a phylogenetic analysis,the MsGLK family members were divided into 13 groups.A collinearity analysis detected 193 MsGLK gene duplicates in the alfalfa genome,and a analysis of the ratio of the number of nonsynonymous substitutions per nonsynonymous site to the number of synonymous substitutions per synonymous site(Ka/Ks)revealed that most of the duplicated gene pairs have undergone purification selection.A range of cis-acting elements were detected in MsGLK gene promoter regions,and were involved in plant growth and development,hormone responses,and stress responses.Analyses of gene transcript profiles revealed that 12 MsGLKs showed tissue-specific expression patterns,and 25 MsGLKs were expressed in all tissues.Further RT-qPCR analyses revealed that some MsGLK genes were activated to some degree in response to drought stress,salt stress,and exogenous ABA treatment.The results of this study provide reference information for further research on the function of MsGLK genes and for the genetic improvement of stress resistance in alfalfa.关键词
紫花苜蓿/GLK基因/生物信息学/渗透胁迫/表达模式Key words
alfalfa/GLK gene/bioinformatics/osmotic stress/expression patterns引用本文复制引用
马超,孙熙婧,冯雅岚,周爽,琚吉浩,吴毅,王添宁,郭彬彬,张均..紫花苜蓿GLK基因家族鉴定及渗透胁迫下的表达分析[J].草业学报,2025,34(1):174-190,17.基金项目
国家自然科学基金(32372227),河南省高等学校青年骨干教师培养计划(2021GGJS050),河南省自然科学基金(242300421319)和河南省科技研发计划联合基金项目(232103810021)资助. （32372227）
