草业学报2025，Vol.34Issue(1)：203-214,12.DOI:10.11686/cyxb2024074
不同乳酸菌制剂组合对油菜秸秆发酵品质和体外瘤胃发酵特性的影响
Effects of different Lactobacillus combinations on fermentation quality and in vitro rumen fermentation characteristics of rape straw
摘要
Abstract
This experiment was designed to investigate the effects of Lactobacillus plantarum and Lactobacillus brucei on the conventional nutrient composition and protein fraction of rape(Brassia campestris)straw,and in vitro rumen fermentation parameters in yaks.Four treatment groups with five replicates in each group were set up,including a control group(CON;without any bacterial preparation),an LP group(with 1.0 g·kg-1 L.plantarum),an LB group(with 1.0 g·kg-1 L.brucei),and an LPB group(with 1.0 g·kg-1 L.plantarum+1.0 g·kg-1 L.brucei).Rape straw was used as fermentation substrate.The rape straw was cut to 2 cm lengths and mixed well with the bacterial preparation,then packed into polyethylene bags.The bags were evacuated and anaerobically fermented at room temperature for 45 days.Samples were taken to determine the nutrient composition,Cornell net carbohydrate and protein(CNCPS)system data,and yak in vitro rumen fermentation parameters of the straws.The results showed that:1)Compared with the CON group,the pH values of the LP,LB,and LPB groups were decreased(P<0.05),and lactic acid content was increased(P<0.05),with the highest values recorded in the LPB group.2)Compared with the CON group,the neutral detergent fiber(NDF),acid detergent fiber(ADF),and cellulose content of the LP,LB,and LPB groups were decreased,and the ether extract content was increased(P<0.05).Furthermore,the NDF,ADF,and acid detergent lignin levels(ADL)in the LPB group were lower than those in the other two treatment groups(P<0.05).3)Compared with the CON group,the rapidly degraded protein(PB1)and moderately degraded protein(PB2)in each treatment group did not change significantly(P>0.05).The content of non-protein nitrogen(PA1)in the LPB group increased(P<0.05),and the content of unavailable protein(PC)decreased(P<0.05).The content of unavailable fiber(CC)in each treatment group decreased(P<0.05),and the content of available fiber(CB2)and non-structural carbohydrates(NSC)increased(P<0.05),compared to CON.The content of CC in the LPB group was lower than that in CON,LP and LB groups(P<0.05).4)After 72 hours in vitro gas production of rape straw,there was no significant difference in rumen fluid pH among the groups(P>0.05).The NH3-N content in each group was lower than that in the CON group(P<0.05).The dry matter degradation rate(DMD)and volatile fatty acid(VFA)content in the LPB group were higher than those in the CON group(P<0.05).In conclusion,the anaerobic fermentation of rape straws inoculated with bacterial preparations improved the nutritional value of the rape straws,and different bacterial preparations differed in their efficacy.On a multivariate comparative score,the treatments ranked:L.plantarum+L.brucei>L.plantarum>L.brucei.关键词
油菜秸秆/康奈尔净碳水化合物蛋白质体系组分/菌制剂/体外发酵Key words
rape straw/Cornell net carbohydrate and protein system/bacterial preparation/in vitro fermentation引用本文复制引用
马永霞,代程程,吴建平,张康林,史海涛,黄艳玲..不同乳酸菌制剂组合对油菜秸秆发酵品质和体外瘤胃发酵特性的影响[J].草业学报,2025,34(1):203-214,12.基金项目
四川省科技厅重点研发项目:油菜秸秆饲料化高效利用技术研究与应用(2022YFN0063),四川肉牛创新团队建设项目(sccxtd-2023-13)和牦牛高效养殖关键技术与集成应用研究资助. （2022YFN0063）
