磁共振成像2024，Vol.15Issue(12)：143-149,7.DOI:10.12015/issn.1674-8034.2024.12.021
IDEAL-IQ序列定量评估2型糖尿病大鼠腹腔实质脏器脂肪沉积及铁过载的应用研究
Application of IDEAL-IQ to quantitatively evaluate fat deposition and iron overload in abdominal parenchymal organs in rats with type 2 diabetes mellitus
摘要
Abstract
Objectives:The MRI iteraterative decomposition of water and fat with echo asymmetry and least-squares estimation quantitation(IDEAL-IQ)technique was utilized to non-invasively and quantitatively assess fat deposition and iron deposition in the liver,kidney and pancreas of rats with type 2 diabetes mellitus(T2DM),as well as to study the relationship between fasting blood glucose(FBG),body weight,and fat deposition and iron deposition in T2DM rats,and to observe the laboratory and pathological alterations between groups.Materials and Methods:Ten specific pathogen free(SPF)healthy male SD rats were randomly grouped into subgroups,experimental group(n=7)and control group(n=3).The experimental group was subjected to the establishment of a model of T2DM,after the experimental group was modeled,the two groups of rats were scanned with MRI IDEAL-IQ.The proton density fat fraction(PDFF)and transverse relaxation rate(R2*)of the liver,pancreas and kidney of the two groups of rats were measured to evaluate the fat deposition and iron overload in the liver,pancreas and kidney of the experimental group and the control group,and to assess the changes in liver function,renal function,and lipids by blood sampling from the heart at the end of the scanning process.The liver,kidney,and pancreas were taken at execution for routine HE staining to observe cellular changes,oil red O staining to observe fat deposition,and Prussian blue iron staining to observe iron deposition.The experimental data were statistically analyzed using SPSS 27.0 software,and the Pearson correlation coefficient was used to analyze the correlation between FBG,body weight and PDFF and R2* values of various organs in rats.Results:The FBG,body weight,triglycerides(TG),and low-density lipoprotein cholesterol(LDL-C)of SD rats in the T2DM group were higher than those of the control group,and the PDFF of the pancreas,liver,right kidney,and left kidney as well as the R2* of the pancreas and liver were higher than those of the control group,and the differences were statistically significant(P<0.05).However,the differences in T1 signal intensity and T2 signal intensity of the pancreas,liver,and both kidneys were not statistically significant between the two groups of rats,and the differences in R2*,total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),aspartate transaminase(AST),alanine aminotransferase(ALT),blood urea nitrogen(BUN),and creatinine(Cr)of both kidneys were not statistically significant when compared with those of the control group(P>0.05).Pearson's correlation analysis showed that the differences between FBG and PDFF in the liver(r=0.773),PDFF of the pancreas(r=0.837),PDFF of the right kidney(r=0.895),PDFF of the left kidney(r=0.784),R2* of the liver(r=0.876),and body weight(r=0.980)were positively correlated(P<0.05).Body weight was positively correlated with PDFF of the pancreas(r=0.840),PDFF of the right kidney(r=0.854),PDFF of the left kidney(r=0.796),PDFF of the liver(r=0.834),and PDFF of the pancreas(r=0.778)(P<0.05).Conclusions:In this experiment,MRI IDEAL-IQ technology was used to non-invasively and quantitatively evaluate the content of fat deposition and iron deposition in the liver and pancreas of T2DM rats,and the difference in fat content in both kidneys of the two groups of rats was also evaluated.This technique is expected to provide a new direction for clinical diagnosis and treatment by dynamically following newly diagnosed diabetes mellitus patients,and assessing changes in liver,kidney,and pancreatic fat and iron content at an early stage.关键词
磁共振成像/IDEAL-IQ/大鼠/2型糖尿病/脂肪沉积/铁沉积Key words
magnetic resonance imaging/IDEAL-IQ/rats/type 2 diabetes mellitus/fat deposition/iron deposition分类
医药卫生引用本文复制引用
倪艳辉,张小明,肖波..IDEAL-IQ序列定量评估2型糖尿病大鼠腹腔实质脏器脂肪沉积及铁过载的应用研究[J].磁共振成像,2024,15(12):143-149,7.基金项目
2024 Chongqing Science and Health Joint Medical Research Project(No.2024MSXM165). 2024年重庆市科卫联合医学科研项目(编号:2024MSXM165) （No.2024MSXM165）
