土壤含水量对玫瑰茄花青素含量及DFR基因表达水平的影响OA北大核心
Effect of soil water content on anthocyanin level and DFR gene expression in roselle
[目的]分析土壤含水量对玫瑰茄萼片花青素含量以及二氢黄酮醇-4-还原酶(DFR)基因表达水平的影响,为玫瑰茄栽培时的水分管理以及进一步明确DFR基因在花青素合成中的功能提供参考.[方法]以玫瑰茄品种'广东早熟'为试验材料,分别设置了 33%(极度干旱)、55%(轻度干旱)、80%(渍涝)3 个土壤含水量处理,分别测定各处理下开花后 10(前期)、20(中期)、30 d(后期)玫瑰茄萼片中花青素的含量以及 5 个DFR基因的表达量,并对花青素含量与DFR基因表达量的相关性进行了分析.[结果](1)不同土壤含水量下,随着开花时间的推移,玫瑰茄萼片花青素含量均呈下降趋势,且开花前期与开花后期的差异显著;含水量为 80%时,花青素含量下降幅度最大;开花前、中期,土壤含水量为 33%的花青素含量明显低于土壤含水量为 55%、80%的含量;开花后期,3 个土壤含水量处理的花青素含量差异不显著.(2)不同土壤含水量下,DFR基因的表达量随着开花时间的推移呈现不同的变化趋势.土壤含水量为 33%时,5 个DFR基因的表达量高于其他两个含水量处理,且在开花后 30d显著上调,达到最高值,其中,DFR4 表达量最高,DFR5 表达量最低;土壤含水量为55%、80%时,5 个DFR基因的表达量均较低.(3)土壤含水量为 55%时,DFR4、DFR5 表达量与花青素含量显著相关,表明其为调控花青素合成的关键基因.[结论]严重的干旱和渍涝都不利于玫瑰茄萼片花青素的合成;干旱胁迫促进了DFR基因的表达,而渍涝情况下DFR基因的表达受到了抑制;DFR4、DFR5 基因为调控玫瑰茄花青素合成的关键基因.
[Objective]The effect of soil water content(SWC)on the anthocyanin content and expression level of dihydroflavonol 4-reductase(DFR)related genes in the calyxes of Hibiscus sabdariffa L.(roselle)were analyzed to provide basis for the water man-agement during roselle cultivation and further clarification of the function of DFR in anthocyanin synthesis.[Method]The seedlings of'Guangdong early-maturity'were treated with 3 levels of SWC,namely 33% (severe drought),55% (moderate drought)and 80% (flood),respectively,and the anthocyanin content as well as the expression levels of 5 DFR genes in the calyxes were meas-ured at 10(early flowering stage),20(middle flowering stage)and 30(late flowering stage)days since flowering.Moreover,the correlation between anthocyanin content and the expression level of DFR genes were analyzed.[Result](1)Under each treatment,the anthocyanin content of roselle calyxes showed decreasing trends with the course of flowering,and varied significantly between the early and late flowering stage,with the sharpest decline occurring at 80% SWC.During early and middle flowering stage,the antho-cyanin contents in the calyxes of roselle were significantly lower at 33% SWC than those of 55% and 80%;while at late flowering stage,the difference in anthocyanin content among the 3 treatments was insignificant.(2)The expressions of DFR genes followed various trends with flowering in response to different SWC treatments.At 33% SWC,the expression levels of the 5 DFR genes were higher than the other 2 treatments,significantly up-regulated,and peaked at 30 d after flowering,among which the expression level of DFR4 ranked the top while that of the DFR5 was at the bottom.Moreover,the expression levels of all the 5 DFR genes were rela-tively low at 55% and 80% SWC.(3)At 55% SWC,the expression levels of DFR4 and DFR5 were significantly correlated with the anthocyanin contents in roselle calyxes,indicating their key roles in regulating anthocyanin synthesis in roselle.[Conclusion]Severe drought and flood impede the synthesis of anthocyanins in roselle calyxes;the expression levels of DFR were promoted by drought stress,while inhibited under waterlogging conditions.In addition,DFR4 and DFR5 genes were the key genes regulating anthocyanin synthesis in roselle calyxes.
汪桦燕;李静;陈浩楠;林荔辉;方平平;徐建堂;张立武;陶爱芬
福建农林大学教育部作物遗传育种与综合利用重点实验室/福建省作物设计育种重点实验室,福建 福州 350002北京林业大学林木遗传育种全国重点实验室,北京 100083
农业科学
玫瑰茄土壤含水量花青素二氢黄酮醇-4-还原酶基因表达
rosellesoil water contentanthocyanindihydroflavonol-4-reductasegene expression
《福建农林大学学报（自然科学版）》 2025 (001)
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现代麻类产业技术体系建设项目(CARS-16-E06);福建省南方麻类种质资源共享平台项目(PTJH20006).
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