福建农林大学学报（自然科学版）2025，Vol.54Issue(1)：100-109,10.DOI:10.13323/j.cnki.j.fafu(nat.sci.).202312028
猪源性肠球菌耐药性与抗锌性的相关性
Correlation analysis between drug and zinc resistance of porcine Enterococcus
摘要
Abstract
[Objective]The porcine Enterococcus resistance to heavy metal zinc and drug,its epidemiological feature and potential cor-relation between zinc and drug resistance genes were analyzed to provide reference for the use of antimicrobial drugs and ZnO in breed-ing.[Method]Enterococcus were isolated from 395 pig stool samples with specific selection medium,and their sensitivity to 9 antimi-crobial drugs and ZnSO4 were determined by microdilution method.Meanwhile,the presence of drug and zinc resistance genes were i-dentified through PCR.[Result]A total of 348 Enterococcus isolates were obtained,with an isolation rate of 88.10% .The drug suscep-tibility testing of Enterococcus isolates showed the highest resistance to erythromycin at 96.81% and the lowest to vancomycin at only 0.71% .Multidrug resistance analysis revealed that Enterococcus isolates with quadruple resistance accounted for the highest proportion(31.91% ).The ZnSO4 sensitivity testing indicated that 52.13% of the isolates had a minimum inhibitory concentration(MIC)≥12 mmol·L-1.The zinc resistance gene zntA had the detection rate of 37.93% .The detection rate of gyrA gene encoding the A subunit of DNA gyrase was 92.79%,while that of macrolide resistance gene mefA was only 0.31% .Correlation analysis indicated that zinc resist-ance gene was significantly positively correlated(P＜0.05)with the tetS gene encoding ribosome protection protein and tetracycline ef-flux gene tetL,but negatively correlated with the tetracycline efflux gene tetG(P＜0.05),gyrA and rRNA methylase gene ermB.[Con-clusion]The heavy metal zinc may induce the development of resistance and multidrug-resistant phenotypes in enteric Enterococcus of swine,and antimicrobial drugs may also influence the zinc resistance of Enterococcus in pig feces.关键词
猪源肠球菌/耐药性/耐药基因/抗锌性/氧化锌Key words
porcine Enterococcus/drug resistance/resistance gene/zinc resistance/zinc oxide分类
农业科技引用本文复制引用
宋辉辉,孙志华,洪娟,赖华敏,任子宁,易灵娴,林欣,俞道进..猪源性肠球菌耐药性与抗锌性的相关性[J].福建农林大学学报（自然科学版）,2025,54(1):100-109,10.基金项目
福建省现代生猪产业技术体系项目(KKE19011A). （KKE19011A）
