康复学报2024，Vol.34Issue(6)：611-616,6.DOI:10.3724/SP.J.1329.2024.06012
基于BDNF/TrkB信号通路探讨高频重复经颅磁刺激对老年小鼠术后认知功能障碍的影响
Effect of High Frequency Repetitive Transcranial Magnetic Stimulation on Aged Mice with Postoperative Cognitive Dysfunction Based on BDNF/TrkB Signaling Pathway
摘要
Abstract
Objective To explore the effect and its mechanism of high frequency repetitive transcranial magnetic stimulation(rTMS)on aged mice with postoperative cognitive dysfunction(POCD).Methods A total of twenty-four 18-month-old C57BL/6J male aged mice were randomly divided into control group,operation group and rTMS group,with eight cases in each group.The rT-MS group received 5 Hz rTMS real stimulation for 10 consecutive days before operation,then underwent an experimental laparoto-my,and continued with another four days of rTMS real stimulation after operation,once a day,with a magnetic stimulation intensity of 0.84 T,frequency of 5 Hz,100 pulses per sequence,a 30-second interval time,10 repetitions for a total of 1 000 pulses.The con-trol group and the operation group received sham stimulation for 10 days before operation,the control group did not undergo the op-eration,while the operation group received experimental laparotomy,and then the control group and the operation group received sham stimulation for four days.Open field test was used to evaluate motor function(percentage of time spend in the central area,to-tal distance traveled,and average speed of movement)at the 3rd day after operation;Y-maze test was used to evaluate cognitive function(the percentage of entries into the novel arm,the percentage of time to stay in the novel arm and the total distance moved in the novel arm)at the 4th day after operation;Western blot was used to detect the protein expression levels of brain-derived neuro-trophic factor(BDNF),tropomyosin related kinase B(TrkB)and nerve growth factor(NGF)in the hippocampal tissue of the mice.Results(1)Motor function:there were no significant differences in the percentage of time spent in the central area of the open field,total distance traveled and average speed of movement among the three groups(P>0.05).(2)Cognitive function:there was no significant difference in the percentage of entries into the novel arm among the three groups(P>0.05);compared with the control group,the percentage of time spent in the novel arm and the total distance moved in the novel arm in the operation group de-creased significantly,and the differences were statistically significant(P<0.05).Compared with the operation group,the percentage of time spent in the novel arm and the total distance moved in the novel arm in the rTMS group increased significantly,and the dif-ferences were statistically significant(P<0.05).(3)Protein expression level of hippocampal tissue:compared with the control group,the protein expression levels of BDNF,TrkB and NGF in the hippocampal tissue in the operation group decreased significantly,and the differences were statistically significant(P<0.05).Compared with the operation group,the protein expression levels of BDNF,TrkB and NGF in the hippocampus in the rTMS group increased significantly,and the differences were statistically significant(P<0.05).Conclusion High frequency rTMS can improve POCD in aged mice,and its mechanism may be related to rTMS activating BDNF/TrkB signaling pathway and increasing NGF expression.关键词
术后认知功能障碍/重复经颅磁刺激/脑源性神经营养因子/原肌球蛋白相关激酶B/神经生长因子Key words
postoperative cognitive dysfunction/repetitive transcranial magnetic stimulation/brain derived neurotrophic fac-tor/tropomyosin receptor kinase B/nerve growth factor引用本文复制引用
李敏,杨孝,任露露,吴庭楣,张弘..基于BDNF/TrkB信号通路探讨高频重复经颅磁刺激对老年小鼠术后认知功能障碍的影响[J].康复学报,2024,34(6):611-616,6.基金项目
国家自然科学基金项目(82172544) （82172544）
上海市虹口区"临床医学优秀青年人才培养"项目(HKYQ2022-06) （HKYQ2022-06）
上海市第四人民医院科研启动专项(sykyqd01001) （sykyqd01001）
