分析化学2024，Vol.52Issue(12)：1844-1852,中插1-中插2,11.DOI:10.19756/j.issn.0253-3820.241332
异喹啉类生物碱与鸟嘌呤-鸟嘌呤-腺嘌呤型三链DNA相互作用的质谱与光谱学研究
Investigation of Interaction of Isoquinoline Alkaloids with Guanine-Guanine-Adenine Triplex DNA by Mass Spectrometry and Spectroscopy
摘要
Abstract
The interaction of several small-molecule alkaloids,namely coptisine,jateorhizine,epiberberine,berberrubine,columbamine,fangchinoline and tetrandrine,with guanine-guanine-adenine(GGA)triplex DNA was investigated by mass spectrometry(MS),ultraviolet-visible(UV-vis)spectroscopy,fluorescence spectroscopy,and circular dichroism(CD)spectroscopy.The effect of the 7 kinds of metal ions on the stability of GGA triplex DNA was investigated by direct infusion electrospray ionization mass spectrometry(ESI-MS),and the binding degree was highest when Ni2+was employed.Among the 7 kinds of alkaloids,tetrandrine had the highest affinity to the GGA triplex.All the data unequivocally accommodated diverse mechanisms for interaction between the isoquinoline alkaloids and the GGA DNA triplexes.At least,electrostatic attraction and intercalative-binding modes were observed in the study.Interestingly,UV melting denaturation assay revealed that the two isoquinoline alkaloids including columbamine and berberrubine enhanced the stability of the GGA triplex structure.The UV-melting curve profile could not accurately determine the stabilizing effect of small molecules on the structure of GGA triplex DNA.Further detection by UV-vis spectroscopy showed that epiberberine could stabilize the structure of GGA triplex DNA,but other small molecules couldn't stabilize the structure of GGA triplex DNA.The mode of interaction was further investigated,and firstly,fluorescence spectroscopy was applied to construct the ethidium bromide(EB)-GGA triplex DNA system,and then the reactions of small molecule alkaloids at different concentrations with EB-GGA triplex DNA system were studied.And it was found that epiberberine,fangchinoline and tetrandrine small molecules could replace the EB and insert to bind GGA triplex DNA.Finally,the circular dichroism spectroscopy was used to investigate the binding mode,and the negative CD signal was found.The investigation provided new insights into the interaction of isoquinoline alkaloids with GGA triplex DNA.关键词
鸟嘌呤-鸟嘌呤-腺嘌呤型三链DNA/生物碱/质谱法/紫外可见光谱/荧光光谱/圆二色光谱Key words
Guanine-guanine-adenine triplex DNA/Alkaloid compounds/Mass spectronietry/Ultraviolet-visible spectroscopy/Fluorescence spectroscopy/Circular dichroism spectroscopy引用本文复制引用
于欣灵,谢兆阳,吴怡,马德来,张哲,杨洪梅..异喹啉类生物碱与鸟嘌呤-鸟嘌呤-腺嘌呤型三链DNA相互作用的质谱与光谱学研究[J].分析化学,2024,52(12):1844-1852,中插1-中插2,11.基金项目
吉林省科技厅中青年科技创新创业卓越人才(团队)项目(No.20220623026TC)和长春中医药大学大学生"金种子"重点支持领域创新创业项目(No.JZZ202309)资助. Supported by the Science and Technology Development Planning Project of Jilin Province(No.20220623026TC)and the"Golden Seed"Key Support Area Innovation and Entrepreneurship Project for College Students for Changchun University of Chinese Medicine(No.JZZ202309). （团队）
