分析化学2024，Vol.52Issue(12)：1865-1876,中插3-中插10,20.DOI:10.19756/j.issn.0253-3820.241268
心肌梗死及心肌梗死后心力衰竭患者血浆酰基肉碱的靶向代谢组学研究
A Targeted Metabolomic Study of Plasma Acylcarnitines in Patients with Myocardial Infarction and Heart Failure after Myocardial Infarction
摘要
Abstract
A combined full-scan mass spectrometry/data-dependent acquisition(Full MS/ddMS2)and full-scan mass spectrometry/parallel reaction monitoring(Full MS/PRM)approach for qualitative and quantitative analysis of plasma acylcarnitines was established on the basis of ultra-performance liquid chromatography-high resolution mass spectrometry(UPLC-HRMS).A total of 165 kinds of acylcarnitines were identified,and 111 kinds of acylcarnitines were quantified.The method demonstrated good reproducibility,with a relative standard deviation of less than 20%for plasma acylcarnitine content.Statistical analyses,including t-tests,partial least squares-discriminant analysis,variable importance in projection and volcano plot analysis,indicated significant alterations in plasma acylcarnitine levels in heart failure patients after myocardial infarction,for instance,46 kinds of acylcarnitines showing marked increases compared to myocardial infarction patients.Acylcarnitines showed a strong positive correlation with the clinical biomarker NT-proBNP,which was associated with heart failure.The combined use of acylcarnitine markers AC(C14-OH),AC(C12:1a),long-chain acylcarnitines,total acylcarnitines and NT-proBNP significantly enhanced the diagnostic sensitivity and specificity for post-infarction heart failure,with the area under the receiver operating characteristic curve(AUC)reaching 0.9456.The results of this work indicated that acylcarnitine was poised to become a crucial metabolic target for precise diagnosis,treatment and nutritional intervention in the progression of heart failure after myocardial infarction.关键词
酰基肉碱/超高效液相色谱-高分辨质谱联用/心肌梗死/心力衰竭/代谢组学Key words
Acylcarnitines/Ultra-performance liquid chromatography-high resolution mass spectrometry/Myocardial infarction/Heart failure/Metabolomics引用本文复制引用
夏子淋,杨柱林,吴昊,李慧敏,赵燕,壮可,张宏,易伦朝..心肌梗死及心肌梗死后心力衰竭患者血浆酰基肉碱的靶向代谢组学研究[J].分析化学,2024,52(12):1865-1876,中插3-中插10,20.基金项目
国家自然科学基金项目(No.22174057)和云南省科技厅昆医联合专项项目(No.202201AY070001-257)资助. Supported by the National Natural Science Foundation of China(No.22174057)and the Yunnan Provincial Department of Science and Technology and Kunming Medical University Joint Special Project(No.202201AY070001-257). （No.22174057）
