分析化学2024，Vol.52Issue(12)：1896-1903,8.DOI:10.19756/j.issn.0253-3820.221223
烯丙基活化制备大孔强阳离子交换介质及其在蛋白分离中的应用
Preparation of Macroporous Strong Cation Exchange Chromatography Medium by Allyl Activation and Its Application in Protein Separation
摘要
Abstract
Strong cation exchange mediums have been widely applied in protein purification.In this work,the strong cation exchange chromatography medium was prepared by coupling small molecular ligands through allyl activation commonly used in agarose-based media.The matrix based on the macroporous microspheres was a copolymer of glycidyl methacrylate and ethylene glycol dimethacrylate.The effects of reaction factors on the ligand density were detailedly investigated.The ligand density in the prepared medium was controlled in the range of 0.126-0.370 mol/L(wet gel).Its adsorption performance was evaluated with lysozyme as a model protein.The static binding capacity was in the range of 37.0-81.0 g/L.The maxium dynamic binding capacity reached 37.7 g/L and the maximum lysozyme recovery was 98.97%.The strong cation exchange medium obtained here showed low non-specific adsorption and high separation efficiency on lysozyme.This strong cation exchange medium showed a great potential in rapid protein purification.关键词
强阳离子交换层析介质/烯丙基活化/配基密度/吸附性能/分离纯化Key words
Strong cation exchange chromatography medium/Allyl activation/Ligand density/Adsorption performance/Separation and purification引用本文复制引用
薛彦晓,侯恒扬,公丕胜,靳海波,赵岚,何广湘,张荣月..烯丙基活化制备大孔强阳离子交换介质及其在蛋白分离中的应用[J].分析化学,2024,52(12):1896-1903,8.基金项目
北京市委组织部青年拔尖人才项目(No.ZZB2019001)、中国科学院化学研究所活体分析化学中国科学院重点实验室开放课题项目(No.ACL201903)和北京市自然科学基金项目(No.L192017)资助. Supported by the Youth Top Talent Project of Organization Department of Beijing Municipal Committee(No.ZZB2019001),the Key Laboratory of Analytical Chemistry for Living Biosystem,Chinese Academy of Sciences(No.ACL201903)and the Natural Science Foundation of Beijing City(No.L192017). （No.ZZB2019001）
