分析化学2024，Vol.52Issue(12)：1913-1922,10.DOI:10.19756/j.issn.0253-3820.241114
基于特征肽的高效液相色谱-串联质谱法鉴定沙门氏菌
Identification of Salmonella Using High Performance Liquid Chromatography-Tandem Mass Spectrometry Based on Characteristics Peptides
摘要
Abstract
A qualitative identification method for Salmonella using high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)with multiple reaction monitoring(MRM)mode was established.Eight characteristic peptides were selected,and their MRM parameters were optimized.Furthermore,the performance of this HPLC-MS/MS method in species identification was further evaluated.Experimental results demonstrated that this method exhibited high specificity,enabling accurate differentiation of Salmonella from conventional food-borne pathogens.In a milk matrix,the 50%limit of detection(LOD50)was determined to be 0.35 CFU/25 g.This method was successfully applied to qualitatively identify Salmonellain five artificially contaminated food samples.The method presented here,in contrast to traditional biochemical assays,offered both speed and specificity,providing a novel means for the rapid and accurate identification of Salmonella in food.It held significant implications for the surveillance,prevention,and control of food-borne diseases.关键词
沙门氏菌/特征肽/高效液相色谱-串联质谱法/多反应监测Key words
Salmonella/Peptide biomarkers/High performance liquid chromatography-tandem mass spectrometry/Multiple reaction monitoring引用本文复制引用
陈梦琪,袁慕云,黄成栋,刘婧文,曾广丰,陈文锐,胡松青,凌莉..基于特征肽的高效液相色谱-串联质谱法鉴定沙门氏菌[J].分析化学,2024,52(12):1913-1922,10.基金项目
国家重点研发计划项目(No.2022YFF1100805)资助. Supported by the National Key Research and Development Program of China(No.2022YFF1100805). （No.2022YFF1100805）
