工业微生物2024，Vol.54Issue(6)：44-48,5.DOI:10.3969/j.issn.1001-6678.2024.06.015
不同生长阶段白条双锯鱼肠道微生物菌群结构多样性研究
Study on the Diversity of Intestinal Microflora Structure in Amphiprion Frenatus at Different Growth Stages
摘要
Abstract
Intestinal tract samples of artificially farmed 3-month-old,6-month-old,12-month-old and 18-month-old Amphiprion frenatus were collected in this research.Then,their intestinal DNA was extracted,the V3-V4 region of 16S rDNA was amplified by PCR for high-throughput sequencing,and the differences in the composition of intestinal microbial community structure were analyzed.The results showed that the number of OTU,Chao1 index,Shannon index and Simpson index in the gut of 3-month-old and 18-month-old Amphiprion frenatus were higher than those at other stages,showing richer species diversity.At the phylum level,the main dominant microbial groups in the intestinal tract were Proteobacteria,Firmicutes and Fusobacteria.At the genus level,the main dominant bacteria genera included Photobacterium,Pseudomonas and Achromobacter.This study can provide a theoretical support for the selection of feed for ornamental fish breeding and the development of microbial probiotic reagents.关键词
白条双锯鱼/肠道微生物/高通量测序/观赏鱼Key words
Amphiprion frenatus/intestinal microflora structure/high-throughput sequencing/ornamental fish引用本文复制引用
朱婷,李涛,李恭如,黎双飞..不同生长阶段白条双锯鱼肠道微生物菌群结构多样性研究[J].工业微生物,2024,54(6):44-48,5.基金项目
该项目受深圳市科技计划专项资助(KCXST20221021111206015,KCXFZ20201221173404012). （KCXST20221021111206015,KCXFZ20201221173404012）
