工业微生物2024，Vol.54Issue(6)：210-213,4.DOI:10.3969/j.issn.1001-6678.2024.06.055
毒力基因缺陷型大肠埃希菌O157∶H7毒力和耐药分析
Analysis for Virulence and Drug Resistance of Virulence Gene Defective Escherichia Coli O157∶H7
侯素娟 1房保国 1李前辉2
作者信息
- 1. 淮安市清江浦区疾病预防控制中心,江苏淮安 223000
- 2. 南京医科大学附属淮安市第一人民医院,江苏淮安 223000
- 折叠
摘要
Abstract
In order to understand the difference of pathogenic genes of Escherichia coli O157∶H7 that causes human diseases,a virulence gene defective Escherichia coli O157∶H7 is isolated from the anal swab of a patient with abdominal pain and diarrhea and without other underlying diseases by the identification method combining bacterial phenotype and nucleic acid RT-PCR,and its virulence and drug sensitivity are analyzed.The RT-PCR nucleic acid detection is conducted by Taq Man Array probe microfluidics to detect that the patient has no other gastrointestinal pathogenic bacteria and diarrhea related viruses.The gene defective Escherichia coli O157∶H7 isolated from the anal swab of the patient only carries eae gene and does not carry stx gene,and the strain dose not show drug resistance.The gene defective Escherichia coli O157∶H7,which only carries the eae gene and does not carry the stx gene,can still cause severe clinical symptoms of abdominal pain and diarrhea in humans,but the disease course is relatively short,with no serious fatal complications,and the treatment is relatively easy.关键词
大肠埃希菌/多重PCR/微流控芯片技术/基因缺陷型/eae基因/非耐药株Key words
Escherichia coli/multiplex PCR/microfluidics/gene defective type/eae gene/non-resistant strain引用本文复制引用
侯素娟,房保国,李前辉..毒力基因缺陷型大肠埃希菌O157∶H7毒力和耐药分析[J].工业微生物,2024,54(6):210-213,4.
