环境工程学报2024，Vol.18Issue(11)：3177-3185,9.DOI:10.12030/j.cjee.202312115
不同氮负荷下生物炭强化厌氧氨氧化体系脱氮效能的影响
Effects of nitrogen load on nitrogen removal efficiency of biochar-enhanced anammox systems
摘要
Abstract
To investigate the effects of biochar on the nitrogen removal efficiency of Anammox,batch experiments were conducted.The total nitrogen removal efficiency of the anammox system under three different nitrogen loads(100,300,and 500 mg/L total nitrogen)was examined.The underlying mechanisms were analyzed based on changes in biochar performance before and after reaction,bacterial extracellular polymeric substances secretion levels,and microbial community succession characteristics.Results showed that biochar addition increased the total nitrogen removal efficiency in all groups(TN100,TN300,and TN500)by 13.6％,4.5％and 1.8％,respectively compared with the control group.Additionally,the final nitrate concentration was significantly lower in biochar-amended groups than in the control group.This improvement could be attributed to biochar's electron conductivity and redox properties,which interacted with EPS,promoting extracellular electron transfer and enhancing nitrate reduction,with the most significant effect observed under low nitrogen loads.Analysis of extracellular polymeric substances showed that the TN 100 group with biochar had the highest average extracellular polymeric substances secretion concentration and better sludge structure stability.However,extracellular polymeric substances(EPS)concentration decreased with increasing nitrogen load.This was explained by the higher nitrate accumulation in high nitrogen-loaded anammox systems,leading to increased EPS consumption as an endogenous electron donor during nitrate reduction.High-throughput sequencing and KEGG prediction analysis showed that biochar significantly increased the relative abundance of anammox bacteria Candidatus Kuenenia,as well as the denitrifying bacteria Acinetobacter and Afipia under low nitrogen load conditions.Furthermore,the functional genes for nitrate reductase EC:1.7.5.1 and EC:1.9.6.1 exhibited higher expression levels in the TN 100 group with biochar,revealing the reason for the more significant nitrogen removal efficiency enhanced by biochar under low nitrogen loading conditions.关键词
氮负荷/生物炭/厌氧氨氧化/部分反硝化/微生物群落Key words
nitrogen concentration/biochar/anammox/partial denitrification/microbial consortia分类
资源环境引用本文复制引用
周鹏宇,付静薇,贺逸飞,李倩,陈荣,李玉友..不同氮负荷下生物炭强化厌氧氨氧化体系脱氮效能的影响[J].环境工程学报,2024,18(11):3177-3185,9.基金项目
国家自然科学基金面上资助项目(52270049) （52270049）
陕西省创新能力支撑计划资助项目(2023KJXX-042) （2023KJXX-042）
日本学术振兴会海外特别研究员资助项目(P20794) （P20794）
