河南农业科学2024，Vol.53Issue(12)：43-53,11.DOI:10.15933/j.cnki.1004-3268.2024.12.005
基于非靶向代谢组学的烤烟田间成熟度物质代谢基础研究
Basic Study on Material Metabolism of Flue-cured Tobacco Related to Maturity Based on Non-targeted Metabolomics
摘要
Abstract
To clarify the quantitative indicators of fresh tobacco leaf maturity,Zhongyan 100 was used as the experimental material.Mid-stem leaves with SPAD values of 10-15(S10～15),15-20(S15～20),and 20-25(S20～25)were harvested to explore the metabolomics characteristics,as well as the appearance evaluation,physical properties,chemical composition,aroma content,and sensory quality of their post-cured leaves.The results showed that the post-cured leaves of the S15～20 treatment obtained the optimal quality,including better appearance quality,good physical properties,harmonious chemical components,and the highest total amount of neutral aromatic substances,showing high maturity.According to the partial least squares discriminant analysis(PLS-DA),32 mutual differential metabolites were identified between two comparison groups,including L-tryptophan,gamma aminobutyric acid,geranic acid,pyrrole-2-carboxylic acid,trehalose,kojibiose,nicotinate D-ribonucleoside,1-methyladenosine,sphinganine,melibiitol,indole,scopoletin,18-hydroxycorticosterone,25-hydroxycholesterol,and kaempferide,et al.These differential metabolites might be key differential metabolites during the maturation process of tobacco.Then,a total of 48 significantly differential metabolites were identified across the two groups,including 11 amino acids,7 steroids,6 organic acids,6 sugars,3 alcohols,3 nucleotides,2 flavonoids,1 vitamin,and 9 other metabolites.These significantly differential metabolites were primarily enriched in pathways such as amino acid biosynthesis,ABC transporters,arachidonic acid metabolism,lysine degradation,biosynthesis of phenylpropanoids,and butanoate metabolism,which might play important regulatory roles during the maturation of tobacco leaves.关键词
烤烟/成熟度/SPAD/代谢组学/质量评价Key words
Flue-cured tobacco/Maturity/SPAD/Metabolomics/Quality evaluation分类
农业科技引用本文复制引用
刘腾飞,许自成,史久长,李秋剑,张力,石秋环,李渊博,张雪,许嘉阳,贾玮,韩丹..基于非靶向代谢组学的烤烟田间成熟度物质代谢基础研究[J].河南农业科学,2024,53(12):43-53,11.基金项目
浙江中烟工业有限责任公司科技项目计划(ZJZY2022B002) （ZJZY2022B002）
河南省烟草公司科技项目(2022410000240016) （2022410000240016）
