河南农业科学2024，Vol.53Issue(12)：62-74,13.DOI:10.15933/j.cnki.1004-3268.2024.12.007
黄花蒿TCP转录因子鉴定及其在MeJA处理下的表达模式分析
Identification of TCP Transcription Factors in Artemisia annua and Analysis of Their Expression Patterns under MeJA Treatment
摘要
Abstract
Based on the genome data of Artemisia annua,the TCP gene family members were identified,and their protein physicochemical properties,evolutionary relationships,and cis-acting elements were analyzed.Transcriptome data were used to analyze the expression pattern of TCP transcription factors in Artemisia annua under different light treatments,and qRT-PCR was used to detect their expression under methyl jasmonate(MeJA)treatment.The results showed that a total of 29 TCP genes(AaTCPs)were identified.AaTCPs differed greatly in protein physicochemical properties,in which the number of amino acids and protein molecular mass ranged from 132 to 443 and 14.60 to 47.86 ku,respectively,and the isoelectric points,instability indices,and fat coefficients were in the ranges of 5.67 to 9.99,33.28 to 69.77,and 51.70 to 77.40,respectively,and all of them were nuclear proteins.Phylogenetic relationships showed that AaTCPs could be classified into class Ⅰ(11)and class Ⅱ(18),unevenly distributed on eight chromosomes.The Motif number of AaTCPs ranged from 1 to 7,all of them contained one conserved TCP2 structural domain,and a large number of light-,hormone-,and stress-responsive elements were present in their promoters.Protein interaction predictions revealed tight interactions among the protein members of AaTCPs except for AaTCP12 and AaTCP8.Transcriptome data showed that the expression levels of AaTCPs varied under different light treatments,and the expression of most genes was induced by light.qRT-PCR analysis revealed that the expression of AaTCPs all increased to different degrees under MeJA treatment,showing dynamic changes with the extension of treatment time.In summary,the AaTCP gene family plays a significant role in light regulation and hormone response processes.关键词
黄花蒿/TCP转录因子/蛋白质互作/茉莉酸甲酯Key words
Artemisia annua/TCP transcription factor/Protein interaction/Methyl jasmonate分类
农业科技引用本文复制引用
马琦洋,宋歌..黄花蒿TCP转录因子鉴定及其在MeJA处理下的表达模式分析[J].河南农业科学,2024,53(12):62-74,13.基金项目
浙江省省级创业训练计划项目(S202310344003X) （S202310344003X）
2023浙江省新苗人才计划项目(2023R410004) （2023R410004）
