河南农业科学2024，Vol.53Issue(12)：75-85,11.DOI:10.15933/j.cnki.1004-3268.2024.12.008
地黄枯草芽孢杆菌全基因组测序及其促生作用相关基因研究
The Complete Gene Sequencing of Bacilus subtilis Isolated from Rehmannia glutinosa and Study of Genes Related to Growth-promoting Effects
摘要
Abstract
To investigate the genes associated with the growth-promoting effects of Bacillus subtilis in Rehmannia glutinosa,whole genome sequencing was performed in this study and the genes associated with the growth-promoting effects were discovered through functional annotation.The results showed that the genome size of Bacillus subtilis,an endophytic growth-promoting bacterium of Rehmannia glutinosa,was 4 310 699 bp,with a GC content of 43.44%,and a total of 4 521 predicted coding genes.A total of 3 013,3 354,4 395,206,52,and 212 functional genes were annotated in the clusters of orthologous groups(COG),gene ontology(GO),kyoto encyclopedia of genes and genomes(KEGG),carbohydrate-active enzymes(CAZyme),comprehensive antibiotic resistance database(CARD),and virulence factor database(VFDB),respectively.The annotation results indicated that there were 46 genes related to growth-promoting function,with the majority of these genes involved in phosphorus solubilization,nitrogen fixation,and iron carrier processes.In the meantime,anti-SMASH predicted that a total of 409 genes were enriched into 12 secondary metabolite synthesis gene clusters,with the majority encoding eight repressors,including fengycin,1-carbapen-2-em-3-carboxylic acid,bacillaene,bacilysin,subtilosin A,surfactin,pulcherriminic acid,and bacillibactin.Among these,pulcherriminic acid and bacillibactin had been observed to facilitate the chelation of ferric ions in the surrounding environment.关键词
枯草芽孢杆菌/地黄/全基因组测序/功能注释/内生菌/促生基因Key words
Bacilus subtilis/Rehmannia glutinosa/Whole genome sequencing/Functional annotation/Endophytic bacteria/Growth-promoting gene分类
农业科技引用本文复制引用
腊贵晓,王林林,郭军旗,赵玉龙,郭红霞,贾慧,杨铁钢..地黄枯草芽孢杆菌全基因组测序及其促生作用相关基因研究[J].河南农业科学,2024,53(12):75-85,11.基金项目
河南省现代农业产业技术体系项目(HARS-22-11-G2) （HARS-22-11-G2）
国家现代农业产业技术体系项目(CARS-21) （CARS-21）
河南省重点研发专项(231111110800) （231111110800）
