河南农业科学2024，Vol.53Issue(12)：139-148,10.DOI:10.15933/j.cnki.1004-3268.2024.12.014
不同质量浓度外源ZnONPs对金鱼草耐热性的影响
Effect of Different Concentrations of Exogenous ZnONPs on the Heat Tolerance of Snapdragon
摘要
Abstract
Using snapdragon seedlings as test materials,this study investigated the effects of foliar spraying of different concentrations(0,50,100,200 mg/L)of zinc oxide nanoparticles(ZnONPs)on the growth,chlorophyll content,osmotic regulation substances,and enzyme activity and related substances in the AsA-GSH cycle of snapdragon seedlings under day/night temperatures of 25℃/20℃,35℃/30℃,and 40℃/35℃stress,in order to explore the role of ZnONPs in alleviating heat stress in snapdragon seedlings.The results showed that under high temperature stress,the height,stem thickness,leaf length,leaf width,and biomass of snapdragon significantly decreased.Applying different concentrations of ZnONPs could alleviate the growth inhibition of snapdragon.Under 40℃/35℃stress,compared with the treatment of spraying 0 mg/L ZnONPs,the treatment of spraying 50-200 mg/L ZnONPs increased the total chlorophyll content and carotenoid content of snapdragon by 3.8%—7.7% and 4.9%—11.7%,respectively.The soluble sugar content and proline content of osmotic regulating substances increased by 12.7%—44.8% and 18.2%—32.9%,respectively.Under 40℃/35℃stress,the activities of ascorbate peroxidase(APX),dehydroascorbate reductase(DHAR),monodehydroascorbate reductase(MDHAR),and glutathione reductase(GR)in the AsA-GSH cycle of snapdragon continuously decreased.After spraying different concentrations of ZnONPs,the activities of APX,DHAR,MDHAR,and GR in snapdragon increased by 35.3%—86.3%,13.5%—24.6%,58.5%—81.5%,and 8.6%—19.7%,respectively.The content of ascorbic acid(AsA),glutathion(GSH),ascorbic acid/dehydroascorbate(AsA/DHA),and glutathione/reduced glutathione(GSH/GSSG)increased by 5.9%—11.7%,9.9%—16.5%,14.4%—31.1%,and 19.5%—37.7%,respectively,while the malondialdehyde(MDA)content,relative conductivity,O2-·production rate,and hydrogen peroxide(H2O2)content of snapdragon seedlings continued to decrease.The conclusion is that different treatments with ZnONPs can improve the heat tolerance of snapdragon,among which spraying 100 mg/L ZnONPs treatment has the best effect on improving the heat tolerance of snapdragon seedlings.关键词
金鱼草/高温胁迫/ZnONPs/AsA-GSH/叶绿素/活性氧Key words
Snapdragon/Heat stress/ZnONPs/AsA-GSH/Chlorophyll/Active oxygen分类
农业科技引用本文复制引用
刘丹,毛凤玲,白金鑫..不同质量浓度外源ZnONPs对金鱼草耐热性的影响[J].河南农业科学,2024,53(12):139-148,10.基金项目
宁夏回族自治区高等学校科学研究资助项目(NYG2024034) （NYG2024034）
2020年度宁夏回族自治区高校一流本科专业建设项目(高教高办[2021]7号) （高教高办[2021]7号）
