南京农业大学学报2025，Vol.48Issue(1)：108-120,13.DOI:10.7685/jnau.202312048
黄瓜-酸黄瓜异附加系鉴定、果实长度变异及其分子机制
Identification,fruit length variation and molecular mechanism of Cucumis sativus-C.hystrix alien addition lines
摘要
Abstract
[Objectives]The paper aimed to explore the molecular mechanism of cucumber fruit shortening caused by the introduction of Cucumis hystrix chromosome 6 in alien addition lines(AAL)by analyzing the fruit differences of different C.sativus-C.hystrix A AL,so as to provide theoretical basis for excellent character mining and utilization of cucumber related wild species.[Methods]Molecular markers and Oligo-FISH were used to identify the alien chromosomes in C.sativus-C.hystrix AAL.The fruit traits of cultivated cucumber'Beijingjietou'(CC3)and different C.sativus-C.hystrix AAL were statistically analyzed,paraffin sections and transcriptome sequencing were carried out,and the results of transcriptome sequencing were verified by real-time fluorescence quantitative PCR(RT-qPCR).[Results]A total of 7 different AAL were identified,including monosomic alien additive lines with the C.hystrix chromosome 6(CC-H06),chromosome 8(CC-H08),chromosome 9(CC-H09)and chromosome 10(CC-H10);double monosomic alien additive lines with the C.hystrix chromosomes 6 and 10(CC-H06+H10);and multiple monosomic alien additional lines with the C.hystrix chromosomes 6,7 and 11(CC-H06+H07+H11)and the C.hystrix chromosomes 6,7,10,11,and 12(CC-H06+H07+H10+H11+H12),respectively.Phenotypic investigation showed that the ovary and fruit length of AAL CC-H08,CC-H09 and CC-H10 were all similar to that of CC3,while CC-H06 was significantly shorter than that of CC3,indicating that the introduction of C.hystrix chromosome 6 would lead to the fruit shortening of CC3.The results of paraffin section showed that the fruit cells of CC-H06 were larger than those of CC3,but the number of cells was significantly reduced.Transcriptomic analysis showed that there were 695 and 2 770 differentially expressed genes derived from C.hystrix and CC3 in CC-H06,respectively,which were mainly concentrated in plant hormone signal transduction,zeatin biosynthesis,diterpenoid biosynthesis,tryptophan metabolism and other hormone synthesis pathways.The results of RT-qPCR verification analysis were consistent with those of transcriptome determination,which showed that the results were true and reliable.[Conclusions]The introduction of C.hystrix chromosome 6 could cause the shortening of fruit and the decrease of fruit cell number in CC3.The fruit shortening of CC-H06 was related to the co-regulation of plant hormones such as auxin,cytokinin and gibberellin.关键词
黄瓜/酸黄瓜/异附加系(ALL)/果实/转录组Key words
Cucumis sativus/Cucumis hystrix/alien addition lines(AAL)/fruit/transcriptome分类
园艺学与植物营养学引用本文复制引用
刘紫薇,冯贤波,赵勤政,夏磊,娄群峰..黄瓜-酸黄瓜异附加系鉴定、果实长度变异及其分子机制[J].南京农业大学学报,2025,48(1):108-120,13.基金项目
国家重点研发计划项目(2021YFD1200200) （2021YFD1200200）
江苏省种业振兴"揭榜挂帅"项目(JBGS[2021]070) （JBGS[2021]070）
江苏省自然科学基金青年基金项目(BK20221007) （BK20221007）
