碱胁迫下油莎豆幼苗生理代谢和转录组分析OA北大核心CSTPCD

Cyperus esculentus L.is both an herb oil crop and an applied cash crop for grain,oil,feed and fertilizer with high comprehensive utilization value,furthermore,it has the characteristics of drought re-sistance and salt and alkali tolerance.Identifying the molecular regulation mechanism of alkali resistance of C.esculentus could lay a foundation for its molecular breeding.In this study,the seedlings of C.esculentus were subjected to 100 mmol/L mixed alkali(NaHCO3 and Na2CO3 at 2:1 of mole ratio)stress treatment,and were sampled at 0,3,6,9 and 12 days after treatment.Physiological indicators such as contents of malondialde-hyde(MDA),soluble protein(SP)and proline(Pro),and activities of superoxide dismutase(SOD),per-oxidase(POD)and catalase(CAT)were determined,and transcriptome analysis was performed.The results showed that under alkali stress,SOD and CAT activities increased rapidly in the earlier 6 days and then de-creased gently;POD activity increased rapidly first and then decreased rapidly after 6 days of stress;MDA content increased gradually first and increased rapidly after 6 days;both Pro and SP contents showed gradual upward trends,and the former increased rapidly,while the latter increased relatively slowly.Transcriptome a-nalysis showed that 5 842 differentially expressed genes(DEGs)were identified at 6 days of alkali stress,a-mong which,3 136 genes up-regulated and 2 706 genes down-regulated.GO and KEGG enrichment analysis showed that the DEGs were significantly enriched at the processes of plant hormone signal transduction,starch and sucrose metabolism and so on.In addition,the results of real-time fluorescence quantitative PCR(qRT-PCR)analysis were roughly the same as those of transcriptome analysis,which confirmed the validity of the RNA-seq data.The above results provided important information for further understanding the response mecha-nism of C.esculentus seedlings to alkali stress.