山东农业科学2024，Vol.56Issue(12)：10-15,6.DOI:10.14083/j.issn.1001-4942.2024.12.002
基于转录组分析的甘薯贮藏根淀粉含量相关SNPs开发
Mining of SNPs Related to Starch Content in Sweet Potato Storage Roots Based on Transcriptome Analysis
摘要
Abstract
Sweet potato is an important food and economic crop,and the starch content in its storage roots is a crucial agronomic trait.Mining molecular markers related to starch content is of great significance for accelerating the breeding process.However,sweet potato exhibits self-incompatibility and has a highly hetero-zygous hexaploid genome,which increases the difficulty in developing molecular markers.In this study,high-starch variety Luoxushu 8 and low-starch variety Zhengshu 20 along with six high-starch and six low-starch lines from their hybrid F1 generation were used as materials.Based on transcriptome sequencing analysis com-bined with phenotypic analysis,44 high-starch-specific SNPs were identified,and nine of them were selected for PCR monoclonal analysis.Three loci,chr9.27120209,chr9.27120256 and chr9.13675504,were found to be significantly associated with high starch content,which were further validated in the previously 12 selected materials and other 16 lines from the hybrid population.This study enriched the approaches for developing mo-lecular markers in sweet potato and provided selectable markers for breeding sweet potato varieties with desired storage root starch content.关键词
甘薯/贮藏根/淀粉含量/单核苷酸多态性标记/转录组分析Key words
Sweet potato/Storage root/Starch content/SNPs/Transcriptome analysis分类
农业科技引用本文复制引用
秦桢,侯夫云,王庆美,李爱贤,周媛媛,解备涛,段文学,张海燕,李广华,董顺旭..基于转录组分析的甘薯贮藏根淀粉含量相关SNPs开发[J].山东农业科学,2024,56(12):10-15,6.基金项目
山东省自然科学基金项目(ZR2024MC207) （ZR2024MC207）
山东省重点研发计划项目(2021LZGC025) （2021LZGC025）
泰山产业领军人才工程项目(LJNY202113) （LJNY202113）
国家甘薯产业技术体系项目(CARS-10-GW08) （CARS-10-GW08）
山东省薯类产业技术体系项目(SDAIT-16-04) （SDAIT-16-04）
