基于转录组分析的甘薯贮藏根淀粉含量相关SNPs开发OA北大核心CSTPCD

Sweet potato is an important food and economic crop,and the starch content in its storage roots is a crucial agronomic trait.Mining molecular markers related to starch content is of great significance for accelerating the breeding process.However,sweet potato exhibits self-incompatibility and has a highly hetero-zygous hexaploid genome,which increases the difficulty in developing molecular markers.In this study,high-starch variety Luoxushu 8 and low-starch variety Zhengshu 20 along with six high-starch and six low-starch lines from their hybrid F1 generation were used as materials.Based on transcriptome sequencing analysis com-bined with phenotypic analysis,44 high-starch-specific SNPs were identified,and nine of them were selected for PCR monoclonal analysis.Three loci,chr9.27120209,chr9.27120256 and chr9.13675504,were found to be significantly associated with high starch content,which were further validated in the previously 12 selected materials and other 16 lines from the hybrid population.This study enriched the approaches for developing mo-lecular markers in sweet potato and provided selectable markers for breeding sweet potato varieties with desired storage root starch content.