山东农业科学2024，Vol.56Issue(12)：54-60,7.DOI:10.14083/j.issn.1001-4942.2024.12.008
NaCl胁迫对滇黄精幼苗生长和生理特性的影响
Effects of NaCl Stress on Seedling Growth and Physiological Characteristics of Polygonatum kingianum Coll.et Hemsl.
摘要
Abstract
In this experiment,the seedlings of Polygonatum kingianum Coll.et Hemsl.were used as test materials,the salt content was controlled by container salt pouring method,and the NaCl concentration was set as 50,100,150 and 200 mmol/L,respectively.Taking clear water as control(CK),the changes of growth and physiological indicators of P.kingianum seedlings under different NaCl concentrations were studied,and the stress degree was evaluated by correlation analysis,principal component analysis and membership function method,in order to explore the influence of different concentrations of NaCl on seedling growth and physiologi-cal characteristics of P.kingianum.The results showed that the growth and physiological indicators of P.king-ianum seedlings were obviously different under different NaCl treatments.With the increase of NaCl concentra-tion,the agronomic characters of P.kingianum seedlings showed the downward trend;the content of photosyn-thetic pigments in leaves changed significantly,and the contents of chlorophyll a,chlorophyll b and carote-noids decreased under NaCl stress;the malondialdehyde(MDA)content,proline(Pro)content,superoxide dismutase(SOD)activity and relative conductivity of leaves showed the upward trend,while peroxidase(POD)activity and reduced glutathione(GSH)content increased first and then decreased.Principal compo-nent analysis results showed that stem diameter,fibrous root length,fibrous root diameter,chlorophyll con-tent,SOD activity,MDA content and relative conductivity could be used as the evaluation indicators for P.kingianum under NaCl stress.According to the results of comprehensive evaluation,the stress degree of differ-ent NaCl concentrations to P.kingianum seedlings was sorted from higher to lower as 200 mmol/L NaCl,150 mmol/L NaCl,50 mmol/L and 100 mmol/L NaCl.The research results could provide theoretical and scientific bases for high-quality cultivation of P.kingianum.关键词
NaCl胁迫/滇黄精幼苗/生长指标/生理特性Key words
NaCl stress/Seedlings of Polygonatum kingianum Coll.et Hemsl./Growth indicators/Physi-ological properties分类
农业科技引用本文复制引用
石诗叠,黄明进,王华磊,罗春丽,李金玲,陈松树,李丹丹,梁瑾,刘红昌,罗夫来..NaCl胁迫对滇黄精幼苗生长和生理特性的影响[J].山东农业科学,2024,56(12):54-60,7.基金项目
国家重点研发计划课题"林下生态种植黄精、淫羊藿等中药材产业关键技术研究与应用示范"(2021YFD1601002) （2021YFD1601002）
贵州省农业农村厅种业专项"黄精种质资源收集保存鉴定评价"(黔农计财[2022]10号) （黔农计财[2022]10号）
贵州省中药材现代农业产业技术体系项目(GZCYTX2024) （GZCYTX2024）
贵州省山地农业关键核心技术攻关项目"贵州特色中药材品种选育及生态种植关键技术研究"(GZNYGJHX-2023011) （GZNYGJHX-2023011）
