消毒剂卫可对猪粪污厌氧发酵过程理化指标及微生物群落的影响OA北大核心CSTPCD

To investigate the effects of residual disinfectants on anaerobic fermentation characteristics of pig manure,different concentrations(0,0.02%,0.1%and 0.5%,marked as CK,L,M and H groups,re-spectively)of disinfectant VirkonTM were set to reveal their impacts on the physicochemical indexes and micro-bial community of the fermentation system.The results were as follows.(1)The concentrations of chemical ox-ygen demand(COD),ammonia nitrogen(NH3-N),total nitrogen(TN)and total phosphorus(TP)in-creased with the increase of VirkonTM concentration during anaerobic fermentation,which showed a dose effect.By the end of the reaction,the above indicators in H group increased by 124.37%,48.78%,95.16%and 192.90%,respectively compared to CK.At the end of anaerobic fermentation,the pH value of each experi-mental group increased and tended to be consistent.In addition,the degradation rates of total solids(TS)and volatile solid(VSS)in H group reduced to varying degrees,which were 6.21%and 3.40%lower than those in CK,respectively.(2)VirkonTM could cause significant changes in the composition,diversity and richness of microbial communities.The Ace,Chao and Shannon indexes in H group were significantly lower than those of CK,while the Simpson index showed an opposite response.Before fermentation,the first dominant bacterial group at phylum level was Firmicutes,followed by Proteobacteria,Actinobacteria,Bacteroideta and Chlo-roflexi;after anaerobic fermentation,the relative abundance of Firmicutes decreased,which decreased from 83.45%to 75.28%in H group.The dominant genus were Clostridium_sensu_stricto_1,Turicibacter,Terris-porobacter,Psychobacter,Corynebacterium and Streptococcus before fermentation;after fermentation,Clostridi-um_sensu_stricto_1 decreased in CK,L and M groups,while its relative abundance increased from 28.86%to 30.33%in H group.In addition,other dominant genus such as Turicibacter showed decline in varying degrees.(3)There were significant positive correlations between pH value and NH3-N and phyla such as Chloroflexita and Synergistota.TN was significantly positively correlated with Firmicutes and Actinobacteria.COD and TN clustered together,while NH3-N clustered with pH value first and then with TP.The research results could provide theoretical references for revealing the damage degree caused by excessive use of disinfectants in anae-robic fermentation systems.