山东农业科学2024，Vol.56Issue(12)：98-104,7.DOI:10.14083/j.issn.1001-4942.2024.12.014
6株核桃腐烂病菌的分子鉴定及生物学特性比较
Molecular Identification and Comparison on Biological Characteristics of Six Walnut Canker Pathogens
摘要
Abstract
In order to investigate the specific types and biological characteristics of walnut canker patho-gens,six isolates of walnut canker pathogen with discrepant colony morphologies were jointly identified with multiple genes based on molecular biological methods,and the variations in their pathogenicity and biological characteristics were further explored.The results indicated that based on the phylogenetic tree,the strain 44-9-2B was clustered with Cytospora melnikii with the bootstrap support rate of 100%;the strains 44-1-2C and 51-1-3C were clustered with C.chrsosperma;the strains 49-2-1A,49-9-4D and 53-1-3F were clustered with Valsa sordida.The strain 44-9-2B had the strongest pathogenicity and produced conidia first,followed by strains 44-1-2C and 51-1-3C,while strains 49-2-1A,49-9-4D and 53-1-3F were the last.The results of biological char-acteristics showed that the optimal growth temperature for the six pathogenic strains was 28℃,and the optimal pH value was 6;light had promoting effects on the growth of the six pathogenic strains;but the optimal nitrogen and carbon sources were different for different pathogenic strains.The findings of this study could provide a theoretical basis for prevention and control of the walnut canker pathogens.关键词
核桃腐烂病/分子鉴定/生物学特性/致病性Key words
Walnut canker/Molecular identification/Biological characteristics/Pathogenicity分类
农业科技引用本文复制引用
靳启明,闫成才,王庆朋,王喆,苟长青,王兰,冯宏祖,郝海婷..6株核桃腐烂病菌的分子鉴定及生物学特性比较[J].山东农业科学,2024,56(12):98-104,7.基金项目
新疆联合基金项目"新疆香梨、苹果树腐烂病致灾机理和防控对策研究"(U1903206) （U1903206）
塔里木大学研究生科研创新项目(TDB-SCX202105) （TDB-SCX202105）
南疆重点产业创新发展支撑项目"库尔勒香梨高效无菌授粉技术研究与示范"(2020DB006) （2020DB006）
