饲料研究2024，Vol.47Issue(22)：112-115,4.DOI:10.13557/j.cnki.issn1002-2813.2024.22.020
枣多糖对体外培养巨噬细胞免疫活性的影响
Effect of jujube polysaccharides on immune activity of macrophages cultured in vitro
摘要
Abstract
The experiment aimed to investigate the impact of different concentrations of ethanol-extracted jujube polysaccharides(JP)on the immune activity of macrophages cultured in vitro.The experiment used ethanol concentrations of 60%,70%,80%,and 90%to extract four types of jujube polysaccharides.A 4×4 double-factor experimental design was adopted,with mouse macrophages as the research subjects,to measure the phagocytic activity,proliferation rate,and cytokine secretion levels of macrophages after treatment with four types of jujube polysaccharides(JP60,JP70,JP80,JP90)at different quality concentrations(25,50,100,150 mg/L).A positive control group was set with lipopolysaccharide(LPS),and a blank control group was added with distilled water.The results showed that compared with the blank control group,all experimental concentrations of JP60,JP70,JP80,and JP90 had a promoting effect on the phagocytic activity,proliferation rate,and the secretion of nitric oxide(NO),tumor necrosis factor-alpha(TNF-α),interleukin-1 beta(IL-1β),and interleukin-6(IL-6)in macrophages.Compared with the LPS control group,the macrophage proliferation rate and phagocytic activity were better in the 100,150 mg/L JP60 groups and the 100,150 mg/L JP90 groups.The 100,150 mg/L JP60 groups and the 50 mg/L JP90 group show better promotion of NO secretion.The study indicates that jujube polysaccharides(JP)of different quality concentrations can enhance the immune activity of macrophages,with 100 mg/L of JP60 showing the better effect.关键词
枣多糖/体外培养/巨噬细胞/免疫活性Key words
jujube polysaccharide/in vitro culture/macrophages/immune activity分类
农业科技引用本文复制引用
刘行,黄燚森,董财鑫,孙益全,张宏宇,李树鹏..枣多糖对体外培养巨噬细胞免疫活性的影响[J].饲料研究,2024,47(22):112-115,4.基金项目
河北省自然科学基金资助项目(项目编号:C2022204237) （项目编号:C2022204237）
河北省现代农业产业技术体系专项(项目编号:HBCT2024240207,HBCT2024250206) （项目编号:HBCT2024240207,HBCT2024250206）
河北农业大学创新创业培育项目(项目编号:C2024064) （项目编号:C2024064）
