饲料研究2024，Vol.47Issue(22)：116-122,7.DOI:10.13557/j.cnki.issn1002-2813.2024.22.021
鼠李糖乳杆菌TCCC 10035的培养条件优化
Optimization of cultivation conditions for Lactobacillus rhamnosus TCCC 10035
摘要
Abstract
The experiment aimed to optimize the culture conditions for Lactobacillus rhamnosus TCCC 10035.Through single-factor experiments and response surface methodology,the culture conditions for Lactobacillus rhamnosus TCCC 10035 were optimized.The results showed that glucose,manganese sulfate,and vitamin C were important components of the fermentation medium for Lactobacillus rhamnosus TCCC 10035.The response surface results obtained the optimal medium parameters as glucose 25.00 g/L,manganese sulfate 0.32 g/L,and vitamin C 0.24 g/L.Further optimization of the culture conditions led to the best culture conditions:an initial pH value of 6.3,an inoculation volume of 4%,static cultivation for 22 hours,and a cultivation temperature of 37℃.Under these conditions,the number of bacteria obtained reached 5.36×109 CFU/mL,which was a 121%increase compared to before optimization.The study indicates that the optimized medium components and fermentation conditions can increase the number of viable bacteria of Lactobacillus rhamnosus TCCC 10035,which has application value in animal husbandry production.关键词
鼠李糖乳杆菌/高密度培养/活菌数/培养条件优化Key words
Lactobacillus rhamnosus/high-density cultivation/viable bacteria/cultivation conditions optimization分类
农业科技引用本文复制引用
王超凡,王慧慧,胡世伟,赵华,张朝正..鼠李糖乳杆菌TCCC 10035的培养条件优化[J].饲料研究,2024,47(22):116-122,7.基金项目
国家自然科学基金面上项目(项目编号:22278321) （项目编号:22278321）
