色谱2025，Vol.43Issue(1)：22-32,11.DOI:10.3724/SP.J.1123.2024.06007
常压质谱用于脂质识别的研究进展
Progress in applications of ambient ionization mass spectrometry for lipids identification
摘要
Abstract
Lipids are indispensable components of living organisms and play pivotal roles in cell-membrane fluidity,energy provision,and neurotransmitter transmission and transport.Lip-ids can act as potential biomarkers of diseases given their abilities to indicate cell-growth sta-tus.For example,the lipid-metabolism processes of cancer cells are distinct from those of nor-mal cells owing to their rapid proliferation and adaptation to ever-changing biological environ-ments.As a result,the ability to rapidly detect,identify,and monitor lipid components is criti-cal for tracking life-related processes and may enhance cancer diagnosis and treatment efficacy.Mass spectrometry(MS)is regarded to be among the most efficient methods for directly obtai-ning molecular-structural information,and is distinctly advantageous for identifying lipids.Re-cent years have witnessed the emergence of ambient mass spectrometry(AMS),which enables direct analyte sampling and ionization without the need for sample preprocessing.These charac-teristics endow AMS with special advantages for identifying and monitoring lipids.Furthermore,the ongoing development of soft ionization technologies has led to the widespread use of AMS for the detection of complex and diverse lipid molecules.Electrospray ionization(ESI)is a gentle ionization method that can be used to detect medium-to-high-polarity compounds and provide detailed chemical information for lipids by producing a fine mist of charged droplets from a liquid sample.Consequently,a series of ESI-based ionization methods have been devel-oped for fabricating different AMS systems capable of rapidly detecting lipids in a simple man-ner.For example,desorption electrospray ionization(DESI)is among the most extensively employed ambient ionization techniques,and has been used to detect a wide range of samples,including solids,liquids,and gases.DESI involves spraying a charged solvent onto the surface of a sample,after which the solvent is desorbed,the analyte is ionized,and the generated ions are transferred to the detector of the mass spectrometer via a gas plume.DESI can easily and precisely regulate the sampling space,thereby offering a highly effective approach for the in-situ detection of lipids from tissue samples.Additionally,single-cell lipid analysis is limited by small cell volumes,complex cellular matrices,and minimal absolute amounts of analyte.Com-mon detection methods for single cells include flow cytometry and fluorescence microscopy,both of which require fluorescent labeling to detect specific target molecules,which limits de-tection selectivity and reproducibility to some extent.ESI-based single-cell mass spectrometry has emerged as a more-effective method for detecting cellular lipids owing to advantages that include high sensitivity,low sample consumption,high throughput,and multiple-detection ca-pabilities.Moreover,lipid chemical diversity poses a significant challenge for determining struc-tural details.Therefore,AMS-based lipid detection has been augmented with a series of chemi-cal-treatment methods that provide more-comprehensive structural information for lipids.For example,diverse gas-phase dissociation techniques have been used to discriminate between lipid C=C-bond isomers and their sn-positions.Strategies that involve chemically modifying specific target C=C bonds prior to MS detection have also been employed.For example,the Paternò-Büchi(P-B)photochemical reaction oxidizes C=C bonds in unsaturated lipids to form oxetane structures,C=C bonds can be epoxidized to form the corresponding oxaziridines,the N-H aziridination reaction converts C=C bonds into aziridines,and the 1ΔO2 ene reaction adds an OOH group to a C=C bond.In this review,we discuss various environmental ionization techniques for lipid AMS developed over the past five years,with an emphasis on typical chemi-cal strategies used to analyze lipid fine structures.Obtaining a high-coverage,high-sensitivity lipid-detection platform based on AMS remains challenging and requires further in-depth studies despite significant improvements in lipid MS-based detection techniques.关键词
常压质谱/离子化技术/脂质/脂质精细结构/串联质谱Key words
ambient mass spectrometry(AMS)/ionization techniques/lipid/lipid fine struc-ture/tandem mass spectrometry(MS/MS)分类
化学引用本文复制引用
王晓蓉,尹伊颜,欧阳津,那娜..常压质谱用于脂质识别的研究进展[J].色谱,2025,43(1):22-32,11.基金项目
国家自然科学基金(22274012,22474010) （22274012,22474010）
中央高校基本科研业务专项资金(2233300007). National Natural Science Foundation of China(Nos.22274012,22474010) （2233300007）
Fundamental Research Funds for the Central Universities(No.2233300007). （No.2233300007）
