三种单胞藻粉在室内卤虫养殖中的应用OA

In our current study,three commercially produced microalgae powder Phaeodactylum tricornutum,Isochrysis zhanjianggensis and Chromochloris zofingiensis were applied to Artemia culture,to study the optimum culture conditions and evaluate the effect of microalgae on the nutrition quality and enzyme activity of the Artemia biomass.The study result clarified the optimal feeding level for P.tricornutum and I.zhanjianggensis.It also showed that 70 salinity resulted higher survival than that at 30 salinity,and survival rate and body length of Artemia with low stocking density(0.1 L-1)were higher than those with high stocking density(0.5 L-1).When Artemia were cultured at salinity 70 and stocking density 0.1/L in 40 L volume,C.zofingiensis feeding resulted significantly higher Artemia survival rate and body length than other two microal-gae feeding.The nutritional composition of Artemia(in terms of dry mass)were closely related to those of microalgae.The crude fat content of Artemia fed with C.zofingiensis had much higher lipid content(15.9%)and lower crude protein con-tent(50.44%),when compared to those fed with P.tricornutum and I.zhanjianggensis(4.39%and 4.62%for crude fat con-tent,60.22%and 60.91%for protein content,respectively).Artemia fed with P.tricornutum had the highest eicosapen-taenoic acid(EPA)content(9.47 mg/g),while Artemia fed with I.zhanjianggensis had the highest docosahexoenoic acid(DHA)content(0.3 mg/g).Moreover,Artemia fed with C.zofingiensis also resulted the highest total antioxidant capacity,total superoxide dismutase,acid phosphatases and alkaline phosphatase activities of Artemia.