铜业工程Issue(6)：11-17,7.DOI:10.3969/j.issn.1009-3842.2024.06.002
典型铬污染场地铬还原菌的筛选及工程培养基优化
Isolation of Chrome-Reducing Bacteria from Typical Chrome-Contaminated Sites and Optimization of Engineering Medium
摘要
Abstract
The chromium-contaminated sites in plateau area were characterized by high altitude,low oxygen content and poor soil nutri-ents,and the isolation of indigenous high-efficiency chromium-reducing bacteria was crucial for the microbial remediation of chromium-contaminated sites.In this study,four typical chromium-contaminated sites in plateau area were used as research objects,and the indig-enous chromium-reducing bacteria were enriched and domesticated,and the tolerance of the bacteria to Cr(Ⅵ)reached more than 1600 mg/L,and the removal rate of Cr(Ⅵ)reached 71.7%after 65 d of incubation.Three single strains of highly efficient chromium-reducing bacteria were isolated from the enriched and domesticated bacterial solution,namely Leucobacter chromiireducens,Microbacterium sp.and Cellulosimicrobium sp.After 18 d of incubation,the removal rate of 300 mg/L Cr(Ⅵ)by each single bacterium reached more than 94.3%.Comparative study of the chromium reduction ability of chromium-reducing monobacteria and mixed bacteria revealed that three strains mixed bacteria had the best Cr(Ⅵ)removal effect,and the Cr(Ⅵ)removal rate increased by 35.84%after 18 d of cultiva-tion.In view of the fact that low-cost and high-efficiency medium was often used for microbial cultivation in the process of engineering application,and the nutrient source in the engineering medium had a significant effect on the electron transfer and Cr(Ⅵ)reduction of microorganisms,this study optimized the carbon and nitrogen sources in the bacterial engineering medium on the basis of inorganic salts medium.The results showed that,with 4 g/L glucose as the carbon source and 6 g/L dried corn syrup as the nitrogen source,the chromium-reducing bacterium showed relatively excellent remediation effect,and the removal rate of Cr(Ⅵ)reached 78.46%after 18 d of cultivation.关键词
铬污染/铬还原菌/筛选/培养基/优化Key words
chromium pollution/chromium-reducing bacteria/isolation/medium/optimization分类
矿业与冶金引用本文复制引用
李红霞,郑鹏,崔兴兰,王雷,史新悦,孙英春,杨晓莉..典型铬污染场地铬还原菌的筛选及工程培养基优化[J].铜业工程,2024,(6):11-17,7.基金项目
国家重点研发计划项目(2019YFC1805903)资助 （2019YFC1805903）
