畜牧与兽医2025，Vol.57Issue(1)：1-6,6.
牛POLB基因核心启动子鉴定及转录调控分析
Identification and analysis of transcriptional regulation of the core promoter for the bovine POLB gene
摘要
Abstract
The aim of this study was to screen the core promoter of the bovine POLB gene and to identify the transcription factors regulating POLB gene expression.In this study,the 1 751 bp upstream sequence of the CDS region of the bovine POLB gene was used as the template.Five promoter reporter gene vectors containing different truncated lengths were constructed to identify the core promoter region by dual lucifer-ase reporter gene activity assay.Candidate transcription factors within the core promoter region were predicted by the AnimalTFDB 3.0 soft-ware.The transcription factor was then overexpressed in the bovine muscle primary cells to analyze its transcriptional regulation of the bovine POLB gene.The results showed that all the five truncated promoter reporter vectors had transcriptional initiation activity.The AnimalTFDB 3.0 predicted that transcription factor SP1 was able to bind the core promoter region of the POLB gene.The relative fluorescence activity of pGL3-151(-151 to-1 bp)was significantly higher than that of pGL3-551(-551 to-1 bp)(P＜0.001).The pGL3-1351(-1 351 to-1 bp)was significantly higher than pGL3-551(-551 to-1 bp)(P＜0.000 1).The present results indicated that the core promoter re-gions were-151 to-1 bp and-1 351 to-551 bp at the upstream of CDS.Overexpression of transcription factor SP1 in bovine muscle prima-ry cells could significantly reduce the activity of the POLB gene(P＜0.000 1).This study identified the core promoter regions of the bovine POLB gene and found that the transcription factor SP1 was able to regulate the transcriptional activity.These findings provided references for further research on the regulatory mechanism of POLB gene expression.关键词
牛/POLB基因/核心启动子/转录因子/SP1Key words
cattle/POLB gene/core promoter/transcription factor/SP1分类
畜牧业引用本文复制引用
吕世杰,张格阳,王香南,冯亚杰,乔智慧,祁兴山,张子敬,施巧婷,王二耀..牛POLB基因核心启动子鉴定及转录调控分析[J].畜牧与兽医,2025,57(1):1-6,6.基金项目
河南省重大科技专项(221100110200) （221100110200）
河南省中央引导地方科技发展资金项目(Z20221343039) （Z20221343039）
国家肉牛牦牛产业技术体系项目(CARS-37) （CARS-37）
泌阳县国家现代农业产业园建设项目(05) （05）
