畜牧与兽医2025，Vol.57Issue(1)：20-28,9.
地方槐猪和外来瘦肉型猪种9个关联突变位点的遗传变异分析
Genetic analysis of 9 proposed causative variants in local Huai pig and imported lean meat pig breeds
摘要
Abstract
This study was to identify the characteristics of genetic variants associated with important traits such as growth and fattening(MC4R,MSTN),spinal number(NR6A1,VRTN),meat quality traits(PHKG1,PRKAG3),and pathogen infections(SYNGR2 and FUT1)in Chinese local Huai pigs and imported lean meat pigs.Competitive allele specific PCR(KASP)and PCR-RFLP were used for gen-otyping of the variants.Based on the original literature,favorable alleles were identified,and a schematic diagram of the percentage of favora-ble and unfavorable alleles was created.The results were as follows:A certain degree of polymorphism was presented at the NR6A1 gene vari-ant in Huai pigs,with a favorable allele frequency of 0.24.The other sites exhibited a single genotype,among which the variants related to meat quality traits(PHKG1,PRKAG3)were favorable alleles,while the variants related to growth,fattening,and pathogen infection,such as MC4R,MSTN,VRTN,SYNGR2,and FUT1,were unfavorable alleles.Duroc pigs had favorable alleles at loci including MC4R,NR6A1,VRTN,SYNGR2,and FUT1,whereas a certain proportion of unfavorable alleles in the PHKG1 gene existed.Large Yorkshire pigs had favorable alleles at the mutation sites of MC4R,SYNGR2,NR6A1,and VRTN,while other sites were unfavorable alleles.Landrace pigs had unfavorable dominant alleles in the mutation sites of MC4R,MSTN,PRKAG3(p.Va124911e),and SYNGR2.Duroc pigs and Landrace pigs were in a genetic imbalance(P＜0.05)at the VRTN gene and at the MC4R gene.The above results provided useful information for the protection and utilization of Huai pig breeds,and for molecular genetic marker breeding of lean meat pig breeds.关键词
槐猪/瘦肉型猪/因果突变/分子遗传标记Key words
Huai pigs/lean meat type pigs/causative variants/molecular genetic marker分类
畜牧业引用本文复制引用
黄佳怡,张玉宏,许卫华..地方槐猪和外来瘦肉型猪种9个关联突变位点的遗传变异分析[J].畜牧与兽医,2025,57(1):20-28,9.基金项目
福建省自然科学基金项目(2021J011096) （2021J011096）
福建省大学生创新创业训练计划项目(S202311312061) （S202311312061）
