地方槐猪和外来瘦肉型猪种9个关联突变位点的遗传变异分析OA北大核心
Genetic analysis of 9 proposed causative variants in local Huai pig and imported lean meat pig breeds
旨在研究中国本土槐猪和引入瘦肉型猪(杜洛克猪、大约克夏猪、长白猪)在生长育肥相关基因MC4R和MSTN、脊椎数量相关基因NR6A1和VRTN、肉质性状相关基因PHKG1和PRKAG3以及病原感染相关基因SYNGR2和FUT1这8个基因9个SNP位点的遗传变异特征.利用竞争性等位基因特异性PCR(KASP)和聚合酶链反应-限制性片段长度多态性(PCR-restriction fragment length polymorphism,PCR-RFLP)方法对突变位点进行基因分型,根据原始文献对各位点进行有利等位基因判定,并制作各猪种在各位点有利等位基因和不利等位基因的百分比示意图.结果:槐猪在NR6A1基因突变位点存在一定多态性,有利等位基因频率为0.24,其他位点表现为单一基因型,其中,PHKG1、PRKAG3突变位点为有利等位基因,而在MC4R、MSTN、VRTN、SYNGR2和FUT1突变位点为不利等位基因;杜洛克猪在MC4R、NR6A1、VRTN、SYNGR2和FUT1突变位点有利等位基因占优,然而,PHKG1基因不利等位基因仍有一定的比例;大约克夏猪在MC4R、SYNGR2、NR6A1和VRTN基因突变位点有利等位基因占优,而其他位点为不利等位基因;长白猪在MC4R、MSTN、PRKAG3(p.Val249Ile)和SYNGR2这4个基因突变位点不利基因占优;杜洛克猪在VRTN基因、长白猪在MC4R基因突变位点处于遗传不平衡状态(P＜0.05).以上结果为槐猪品种保护与利用以及瘦肉型猪种分子遗传标记选育提供了有益的信息.
This study was to identify the characteristics of genetic variants associated with important traits such as growth and fattening(MC4R,MSTN),spinal number(NR6A1,VRTN),meat quality traits(PHKG1,PRKAG3),and pathogen infections(SYNGR2 and FUT1)in Chinese local Huai pigs and imported lean meat pigs.Competitive allele specific PCR(KASP)and PCR-RFLP were used for gen-otyping of the variants.Based on the original literature,favorable alleles were identified,and a schematic diagram of the percentage of favora-ble and unfavorable alleles was created.The results were as follows:A certain degree of polymorphism was presented at the NR6A1 gene vari-ant in Huai pigs,with a favorable allele frequency of 0.24.The other sites exhibited a single genotype,among which the variants related to meat quality traits(PHKG1,PRKAG3)were favorable alleles,while the variants related to growth,fattening,and pathogen infection,such as MC4R,MSTN,VRTN,SYNGR2,and FUT1,were unfavorable alleles.Duroc pigs had favorable alleles at loci including MC4R,NR6A1,VRTN,SYNGR2,and FUT1,whereas a certain proportion of unfavorable alleles in the PHKG1 gene existed.Large Yorkshire pigs had favorable alleles at the mutation sites of MC4R,SYNGR2,NR6A1,and VRTN,while other sites were unfavorable alleles.Landrace pigs had unfavorable dominant alleles in the mutation sites of MC4R,MSTN,PRKAG3(p.Va124911e),and SYNGR2.Duroc pigs and Landrace pigs were in a genetic imbalance(P＜0.05)at the VRTN gene and at the MC4R gene.The above results provided useful information for the protection and utilization of Huai pig breeds,and for molecular genetic marker breeding of lean meat pig breeds.
黄佳怡;张玉宏;许卫华
龙岩学院生命科学学院,福建龙岩 364012龙岩市农业学校,福建龙岩 364031
畜牧业
槐猪瘦肉型猪因果突变分子遗传标记
Huai pigslean meat type pigscausative variantsmolecular genetic marker
《畜牧与兽医》 2025 (001)
20-28 / 9
福建省自然科学基金项目(2021J011096);福建省大学生创新创业训练计划项目(S202311312061)
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