畜牧与兽医2025，Vol.57Issue(1)：43-49,7.
猪调节性T细胞分子标记物FoxP3单克隆抗体的制备与鉴定
Preparation and identification of monoclonal antibodies of porcine regulatory T cells molecular markers FoxP3
摘要
Abstract
Regulatory T cells(Tregs)are a type of lymphocyte that has immunomodulatory activity,and they induce autoimmune tolerance by inhibiting various effector lymphocytes.FoxP3 gene is an important regulatory gene in the differentiation,development and function main-tenance of CD4+CD25+Treg cells,and it is an important transcription factor.The combination of FoxP3 antigen on the basis of CD4+CD25+can better recognize CD4+CD25+Treg cells.In order to produce specific monoclonal antibody against porcine regulatory T cell surface marker FoxP3,a prokaryotic expression plasmid of FoxP3 epitope(1-840 bp)was constructed here by selecting a gene coding fragment with strong antigenicity.After induction and purification,BALB/c mice were immunized with the target protein,and spleen cells of the immunized mice were fused with myeloma cells,and positive hybrioma cells that could secrete antibodies stably were screened using the indirect ELISA,Western blot and indirect immunofluorescence methods.The results showed that five FoxP3 monoclonal cells with high titer(F4B9,F4A9,F1E12,F1A11 and F4A10)were finally screened and they had good specificity;all of the cells could recognize endogenous and exogenous proteins and secrete antibodies stably,which laid a foundation for qualitative or quantitative detection and verification of the function of Tregs in pigs.关键词
猪/Treg细胞/FoxP3/抗体制备/单克隆抗Key words
porcine/Treg cells/FoxP3/antibody preparation/monoclonal antibodies分类
畜牧业引用本文复制引用
蒋丹,林佳琦,胡鹏程,郭博伦,林新,马燕梅..猪调节性T细胞分子标记物FoxP3单克隆抗体的制备与鉴定[J].畜牧与兽医,2025,57(1):43-49,7.基金项目
福建省自然科学基金项目(2021J01082) （2021J01082）
福建农林大学科技创新专项基金项目(KFb22066XA) （KFb22066XA）
福建农林大学2022年校级大学生创新创业训练计划项目(X202210389094) （X202210389094）
